| UPLC法同时测定人参糖中7种人参皂苷 |
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| 引用本文： | 逄世峰,曲正义,李亚丽,孙成贺,王英平.UPLC法同时测定人参糖中7种人参皂苷[J].特产研究,2014,36(3):53-56. |
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| 作者姓名： | 逄世峰 曲正义 李亚丽 孙成贺 王英平 |
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| 作者单位： | 中国农业科学院特产研究所,长春,130112 |
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| 基金项目： | 吉林省科技支撑计划，国家科技支撑计划项目 |
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| 摘 要： | 建立同时测定人参糖中7种人参皂苷含量的快速检测方法。采用超高效液相色谱法,ACQUITY UPLC BEH C18色谱柱(2.1×50mm,1.7μm),以乙腈-水为流动相,梯度洗脱,流速0.5mL/min,柱温35℃,进样量2μL,检测波长203nm。人参皂苷Rg1、Rf、Re、Rb1、Rc、Rb2、Rd均达到基线分离,线性良好,平均回收率分别为98.26%、98.46%、97.00%、103.75%、96.32%、95.71%、95.00%。该方法准确、重现性好,可用于人参糖中人参皂苷含量测定。
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| 关 键 词： | 超高效液相色谱 人参糖 人参皂苷 |
Determination of Seven Ginsenosides in Ginseng Sweets by UPLC |
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| PANG Shi-feng,QU Zheng-yi,LI Ya-li,SUN Cheng-he,WANG Ying-ping.Determination of Seven Ginsenosides in Ginseng Sweets by UPLC[J].Special Wild Economic Animal and Plant Research,2014,36(3):53-56. |
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| Authors: | PANG Shi-feng QU Zheng-yi LI Ya-li SUN Cheng-he WANG Ying-ping |
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| Institution: | PANG Shi - feng, QU Zheng - yi, LI Ya - li, SUN Cheng - he, WANG Ying - ping (Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun 130112, China) |
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| Abstract: | To develop a method for the content determination of seven ginsenosides in ginseng sweets. This assay was performed on ACQUITY BEH C18（2.1×50mm,1.7μm）column with acetonitrile- water as mobile phase in gradient elution at a flow rate of 0.5mL/min. The column temperature was 35 ℃. The injection volume was 2μL. And the detection wavelength was 203um. Seven compounds were baseline separated,with good linearity.The yield rate was 98.26% ,98.46% ,97.00%, 103.75% ,96.32% ,95.71% ,95.00% .This method is accurate and good reproducibility suitable for the measurement of ginsenosides in the ginseng sweets. |
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| Keywords: | UPLC ginseng sweets gensenosides |
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