水稻转基因再生植株及其后代抗虫性筛选测定研究

采用基因枪转化系统，将CpTI(豇豆胰蛋白酶抑制剂）抗虫基因转入水稻愈伤组织中，经过抗生素筛选得到了再生植株。我们用这些转基因水稻再生植株及其后代的分蘖茎段对玉米螟（Ostrinia nubilalis)进行室内喂养，通过比较虫体死亡率及虫体生长长度，测定转基因水稻再生植株的抗虫性。1997－1999年共进行3个世代5次的室内测定。从121株F0代转基因植株中选出抗虫性较好的植株15株。从F1代的15个株系中，又筛选出了7个株系40个单株；在F2代的14个株系中共选出37株抗虫效果表现较好的植株。对37株中的19株进行了分子检测（PCR扩增），从中检测出5份材料为纯合体，14份材料有分离现象。研究结果表明：该富内喂养玉米螟并进行抗虫测定的方法是可行的；抗虫基因在转基因水稻再生植株中已经获得表达并且可以遗传。

The Selection and Determinaton of the Pest-resistant Character in Transgenic Regenerative Rice Plants and Their Descendances

Using gene gun transformation system, we transformation CpTI (Cowpea Trypsin Inhibitor) gene to rice callus tissues; through anti-biotic selection. The transgenic regenerative rice plants were gotten. Indoor feeding corn borer( Ostrinia nubilalis) by these transgenic rice regenaration plants and their descendance' s tiller stem ; The corn borer mortality and the worm length were compared to determinate transgenic regenerative rice's pest resistance. From 1997 to 1999,5 indoor tests had been taken on three generations. 15 better pest resistance plants were selected from 121 plants in F0 generation, 7 strains 40 better single plants from 15 strains in F1 generation, and 37 better plants from 14 strains in F2 generation. We did molecular test(PCR) to 19 plants from the 37 better plants, and asserted that 5 material are homozygous plants, the other 14 material have segregation. All of these stated that the anti-pest gene in transgenic regenerative rice can express and is heritable.