| 野大麦CIPK基因的亚细胞定位 |
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| 引用本文： | 李率帅,阚国仕,魏建华,李瑞芬. 野大麦CIPK基因的亚细胞定位[J]. 辽宁农业科学, 2011, 0(4): 1-5 |
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| 作者姓名： | 李率帅 阚国仕 魏建华 李瑞芬 |
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| 作者单位： | 沈阳农业大学生物科学技术学院;北京市农林科学院北京农业生物技术研究中心; |
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| 基金项目： | 国家重大转基因专项(2009ZX08009-060B); 国家自然科学基金项目(30971850); 北京自然科学基金项目(5102017); 北京市农林科学院科技项目(2010A003) |
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| 摘 要： | 为明确野大麦CIPK基因在植物亚细胞水平上的定位情况，以已知细胞质膜定位的拟南芥AtCBL1基因和液泡膜定位的AtCBL3基因作为参照，构建了这两个基因分别与红色荧光蛋白基因（RFP）融合的植物表达载体（参照载体），以及野大麦CIPK基因与绿色荧光蛋白基因（GFP）融合的植物表达载体（目标载体），利用基因枪转化法将参照载体和目标载体共转化洋葱上表皮细胞，瞬时表达后利用激光共聚焦显微镜进行共定位分析。结果表明，该基因主要定位于细胞膜和细胞核，为进一步深入分析该基因的功能提供了重要依据。
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| 关 键 词： | 野大麦CIPK基因 基因枪共转化 亚细胞定位 |
The subcellular localization of CIPK from Hordeum brevisubulatum |
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| LI Shuai-shuai,KAN Guo-shi,WEI Jian-hua,LI Rui-fen. The subcellular localization of CIPK from Hordeum brevisubulatum[J]. Liaoning Agricultural Sciences, 2011, 0(4): 1-5 |
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| Authors: | LI Shuai-shuai KAN Guo-shi WEI Jian-hua LI Rui-fen |
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| Affiliation: | LI Shuai-shuai1,KAN Guo-shi1,WEI Jian-hua2,LI Rui-fen2(1.College of Bioscience and Biotechnology,Shenyang Agricultural University,Shenyang 110866,2.Beijing Agro-Biotechnology Research Center,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097) |
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| Abstract: | In order to determine the subcellular localization of CIPK from Hordeum brevisubulatum,using Arabidopsis plasma membrane-localized AtCBL1 and vacuole membrane-localized AtCBL3 as marker genes,two plant expression vectors were constructed as reference vectors,in which these two marker genes were fused with RFP,respectively.The target vector was constructed harbouring CIPK from Hordeum brevisubulatum fused with GFP.The reference and target vectors were co-transformed into onion epidermal cells by particle bom... |
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| Keywords: | Hordeum brevisubulatum CIPK Particle bombardment-mediated co-transformation Subcelluar localization |
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