| 适于SSR的大豆DNA经济高效提取方法 |
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| 引用本文： | 刘春颖,李殿平,孙国伟. 适于SSR的大豆DNA经济高效提取方法[J]. 辽宁农业科学, 2009, 0(1): 49-50 |
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| 作者姓名： | 刘春颖 李殿平 孙国伟 |
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| 作者单位： | 铁岭市农业科学院,辽宁,铁岭,112616;铁岭市农业科学院,辽宁,铁岭,112616;铁岭市农业科学院,辽宁,铁岭,112616 |
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| 基金项目： | 辽宁省自然科学基金项目 |
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| 摘 要： | 在研究大豆遗传多样性的过程中，为了获得清晰、重复性好的SSR扩增结果，DNA的提取起着决定性的作用。以大豆干种子为实验材料，采用改良的SDS法对大豆DNA进行提取。试验结果表明，该改良方法所提DNA凝胶条带清晰，无拖尾现象，OD260／OD280检测值在1．7～1．9之间，用于PCR结果可靠、理想。
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| 关 键 词： | 大豆干种子 DNA提取 改良方法 PCR |
A Cost-effective Extraction Method of Genome DNA from Soybean for SSR |
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| LIU Chun-ying,Li Dian-ping,Sun guo-wei. A Cost-effective Extraction Method of Genome DNA from Soybean for SSR[J]. Liaoning Agricultural Sciences, 2009, 0(1): 49-50 |
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| Authors: | LIU Chun-ying Li Dian-ping Sun guo-wei |
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| Affiliation: | (Academy of Agricuhural Sciences, Tieling City, Liaoning Tieling 112616) |
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| Abstract: | In the study of the soybean genetic diversity, in order to obtain a clear, reproducible results of the SSR amplification, DNA extraction played a decisive role. Soybean seed to dry for the experimental materials, use of improved soybean SDS method for DNA extraction. The results showed that the modified DNA gel method mentioned in the article with clear, non-tailing phenomenon, OD260/OD280 detected in the value between 1.7 to 1.9 for reliable PCR results of the ideal. |
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| Keywords: | PCR |
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