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中性纤维素酶整理牛仔布的工艺优化
引用本文:蔡金涛,焦聪,崔琰,杨佳颖,陈红漫,阚国仕.中性纤维素酶整理牛仔布的工艺优化[J].辽宁农业科学,2011(3):12-15.
作者姓名:蔡金涛  焦聪  崔琰  杨佳颖  陈红漫  阚国仕
作者单位:沈阳农业大学生物科学技术学院,辽宁沈阳,110866
摘    要:根据芽孢杆菌M01-ZL-110所产纤维素酶的酶系组成及酶学性质特点,将该菌株发酵所产纤维素酶酶液应用于牛仔布的整理工艺。以减量率为试验评价指标,通过正交试验对加酶量、温度、整理时间及pH值等参数进行优化。试验结果表明由芽孢杆菌M01-ZL-110制备的中性纤维素酶对牛仔布酶洗整理的最佳条件为:30 m l的溶液体系中酶液加入量为20 m l,pH为6,温度为60℃,整理时间是60 m in,并且对牛仔布表面进行了酶洗前后扫描电镜观察比较。

关 键 词:中性纤维素酶  酶洗  减量率

The Optimum of Process Conditions for Neutral Cellulase Treatment the Denim
CAI Jin-tao,JIAO Cong,CUI Yan,YANG Jia-ying,CHEN Hong-man,KAN Guo-shi.The Optimum of Process Conditions for Neutral Cellulase Treatment the Denim[J].Liaoning Agricultural Sciences,2011(3):12-15.
Authors:CAI Jin-tao  JIAO Cong  CUI Yan  YANG Jia-ying  CHEN Hong-man  KAN Guo-shi
Institution:CAI Jin-tao,JIAO Cong,CUI Yan,YANG Jia-ying,CHEN Hong-man,KAN Guo-shi(College of Biotechnology,Shengyang Agricultural University,Shenyang 110161)
Abstract:According to the cellulase production characteristics of Bacillus Strain M01-ZL110,its crude enzyme extract was applied in denim enzyme wash finishing.Weight loss rate was used as criteria of appraisal,and orthogonal experiment was practised to determine the optimal process conditions.The results revealed that optimum condition as follows.The reaction volume was 30 ml and contained:20 ml of the crude enzyme extract,pH value was 6,10 ml of 0.2 M disodium hydrogen phosphate and 0.1 M sodium citrate buffer sol...
Keywords:Neutral Cellulose  Enzyme Washing  Weight Loss Rate  
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