| 索拉亚百合茎尖组织培养研究 |
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| 引用本文： | 陈颖,王亚斌,罗凤霞,年玉欣,祝朋芳,孙晓梅.索拉亚百合茎尖组织培养研究[J].辽宁农业科学,2005(2):6-8. |
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| 作者姓名： | 陈颖 王亚斌 罗凤霞 年玉欣 祝朋芳 孙晓梅 |
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| 作者单位： | 1. 沈阳农业大学林学院,辽宁,沈阳,110161
2. 北京市农林科学院蔬菜中心,北京,100089 |
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| 摘 要： | 以索拉亚百合的茎尖为外植体，成功建立了快速无性繁殖体系。茎尖愈伤组织诱导培养基为：MS BA3mg／L NAA0．3mg／L；愈伤组织最佳芽分化诱导培养基为：MS BA1．5mg／L NAA0．5mg／L；芽继代增殖的最佳培养基为MS BA1．5mg／L IBA0．05mg／L。KT对芽的增殖效果不如BA，IBA的效果明显好于IAA。此外，激素的比例对不定芽的生长也有影响，最关键的因素是IBA的使用浓度，以0．05mg／L左右为宜。生根培养基为：MS IAA0．5mg／L NAA0．5mg／L AC1mg／L生根率达100％，平均生根数为5．88/苗。移栽成活率可达95％。
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| 关 键 词： | 索拉亚百合 茎尖 组织培养 快速繁殖 |
| 文章编号： | 1002-1728(2005)02-0006-03 |
| 修稿时间： | 2004年5月18日 |
Tissue Culture of the Growing Tip of Lilium hybridum SOLAIA |
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| CHEN Ying,WANG Ya-bin,LUO Feng-xia,NIAN Yu-xin,ZHU Peng-fang,SUN Xiao-mei.Tissue Culture of the Growing Tip of Lilium hybridum SOLAIA[J].Liaoning Agricultural Sciences,2005(2):6-8. |
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| Authors: | CHEN Ying WANG Ya-bin LUO Feng-xia NIAN Yu-xin ZHU Peng-fang SUN Xiao-mei |
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| Institution: | CHEN Ying 1,WANG Ya-bin 1,LUO Feng-xia 2,NIAN Yu-xin 1,ZHU Peng-fang 1,SUN Xiao-mei 1 |
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| Abstract: | The clone propagation of SOLAIA is developed through culture of growing tips in vitro.Induction medium of callus and shoots are MS+BA3mg/L+NAA0.3mg/L,MS+BA1.5mg/L+NAA0.5mg/L.Proliferation medium are MS+BA0.1mg/L+IBA0.05mg/L.Taking proliferation into account,BA is better than KT and IBA is better than IAA.The proportion of hormone also works.The core is the employ-ing of IBA and its concentration,0.05mg/L or so is best.Induction of roots are better in medium of MS+IAA0.5mg/L+NAA0.5mg/L+AC1mg/L.The percentage is100%,5.88roots per shoot and the per-centage of transplantation is95%. |
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| Keywords: | SOLAIA Growing tip Tissue culture Rapid propagation |
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