白头翁叶斑病病原菌生物学研究

对白头翁叶斑病病原菌银莲花壳二孢(Ascochyta anem ones Kab.et Bub.)生物学特性进行了研究。结果表明,病菌菌丝生长的最适培养基为白头翁叶煎汁+PDA,最适温度为20℃,最适pH为6,最适碳源为麦芽糖,最适氮源为蛋白胨;分生孢子器形成的最适培养基为PSA,最适温度为25℃,最适pH为7~9,在黑暗下易于形成分生孢子器,最适碳源为乳糖,最适氮源为甘氨酸;病菌分生孢子萌发最适温度为25℃,pH在7~9较适,在光照下易于萌发,最适碳源为麦芽糖,最适氮源为天门冬氨酸;分生孢子致死温度为47℃,10 m in。

Pathogen Biology of Anemone Leaf Spot

The biological characteristics of Ascochyta anemones Kab.et Bub.were studied.The results indicated that the optimum medium for its growth was leaf decocts of Radix Pulsatillae＋PDA,the optimum temperature was 20 ℃,the optimum pH was 6,the optimum carbon source was Maltose,the optimum nitrogen source was Poptone;the optimum medium for pycnidium formation was PSA,the optimum temperature was 25 ℃,the optimum pH was7～9,dark was beneficial for sporulation,the optimum carbon source was lactase,the optimum nitrogen source was Glycocoll;the optimum temperature for conidia germination was 25 ℃,the optimum pH was 7～9,sunlight was benefical for conidia germination,the optimum carbon source was lactase,the optimum nitrogen source was Aspartic acid;the conidium lethal temperature was 47 ℃ for 10 min.