| 酿酒酵母ADH2基因沉默表达载体的构建 |
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| 引用本文： | 林燕环,魏芳,叶冰莹,张积森,陈由强.酿酒酵母ADH2基因沉默表达载体的构建[J].亚热带农业研究,2011,7(3):197-200. |
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| 作者姓名： | 林燕环 魏芳 叶冰莹 张积森 陈由强 |
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| 作者单位： | 福建师范大学生命科学学院／农业部甘蔗遗传改良重点开放实验室,福建福州,350108 |
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| 基金项目： | 现代农业产业技术体系建设专项资金资助(CARS-20) |
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| 摘 要： | 利用重叠延伸PCR获得酿酒酵母基因沉默组件SADH2,经酶切与酿酒酵母穿梭质粒pYES2.0连接,构建酿酒酵母ADH2基因沉默表达载体。经酶切及测序鉴定成功构建了ADH2基因沉默表达载体pSADH2。
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| 关 键 词： | 酿酒酵母 乙醇脱氢酶 pYES2.0 重叠延伸PCR |
Constructing the alcohol dehydrogenase ADH2 gene silencing expression vector for Saccharomyces cerevisiae |
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| LIN Yan-huan,WEI Fang,YE Bing-ying,ZHANG Ji-sen,CHEN You-qiang.Constructing the alcohol dehydrogenase ADH2 gene silencing expression vector for Saccharomyces cerevisiae[J].Subtropical Agriculture Research,2011,7(3):197-200. |
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| Authors: | LIN Yan-huan WEI Fang YE Bing-ying ZHANG Ji-sen CHEN You-qiang |
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| Institution: | LIN Yan-huan,WEI Fang,YE Bing-ying,ZHANG Ji-sen,CHEN You-qiang(College of Life Sciences,Fujian Normal University/ Key Laboratory of Sugarcane Genetic Improvement,Ministry of Agriculture,Fuzhou,Fujian 350108,China) |
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| Abstract: | It was to synthesize the silencing gene SADH2 of Saccharomyces cerevisiae with overlapping PCR.The silencing gene SADH2 was inserted in pYES2.0 to construct S.cerevisiae ADH2 gene silencing expression vector.Sequencing results showed that the expression vector pSADH2 was correct. |
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| Keywords: | Saccharomyces cerevisiae alcohol dehydrogenase pYES2 0 overlap PCR |
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