| 九里香、柚花瓣HPL基因保守序列的克隆与分析 |
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| 引用本文： | 郭凤芝,姜翠翠,冯会,陈桂信,夏海武,佘文琴,潘东明.九里香、柚花瓣HPL基因保守序列的克隆与分析[J].亚热带农业研究,2007,3(1):73-76. |
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| 作者姓名： | 郭凤芝 姜翠翠 冯会 陈桂信 夏海武 佘文琴 潘东明 |
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| 作者单位： | 1. 福建农林大学园艺植物遗传育种研究所,福建,福州,350002
2. 福建农林大学园艺学院,福建,福州,350002 |
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| 摘 要： | 根据GenBank中登录的一些植物的脂氢过氧化物裂解酶基因的保守序列设计引物,采用RT-PCR技术从芸香科的2种植物(九里香、柚)的花瓣中扩增出该基因的cDNA片段。测序表明,片段大小为512bp。2个片段均包含HPL基因的4个活性中心,并且同源性很高。
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| 关 键 词： | 九里香 柚 脂氢过氧化物裂解酶(HPL)基因 cDNA 克隆 |
| 文章编号： | 1673-0925（2007）01-0073-04 |
| 收稿时间： | 2006-09-18 |
| 修稿时间： | 2006年9月18日 |
Cloning and sequencing of cDNA encoding conservative regions of hydroperoxide lyase in petals of Murraya paniculata, Citrus maxima |
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| GUO Feng-zhi,JIANG Cui-cui,FENG Hui,CHEN Gui-xin,XIA Hai-wu,SHE Wen-qin,PAN Dong-ming.Cloning and sequencing of cDNA encoding conservative regions of hydroperoxide lyase in petals of Murraya paniculata, Citrus maxima[J].Subtropical Agriculture Research,2007,3(1):73-76. |
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| Authors: | GUO Feng-zhi JIANG Cui-cui FENG Hui CHEN Gui-xin XIA Hai-wu SHE Wen-qin PAN Dong-ming |
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| Institution: | 1. Institute of Horticultural Genetics and Breeding;2. College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China |
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| Abstract: | PCR primers were designed based on the consensus sequence of some hydroperoxide lyase(HPL) genes registered in GenBank.Two aimed 512 bp HPL gene-conserving sequences were obtained by using RT-PCR.Both of the fragments contained the four conserved structure domains of HPL gene and shared high homology each other. |
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| Keywords: | Murraya paniculata Citrus maxima hydroperoxide lyase(HPL) gene cDNA clone |
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