Trout leukocytes: growth in oxygenated cultures

A reproducible method of culturing leukocytes from rainbow trout (Salmo gairdneri) has been found. Using an atmosphere of 100 percent oxygen, we stimulated peripheral blood leukocytes to divide at 19 degrees C. Cultured cells were used for a karyological study; they had a modal chromosome number of 60 and a constant arm number of 104, a value previously reported for other tissues in these fish.