| 从棉胚珠衍生细胞培养纤维的研究 |
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| 引用本文： | 孙振元,郑湘如,徐楚年,林庆文.从棉胚珠衍生细胞培养纤维的研究[J].中国农业大学学报,1992(Z1):143-146. |
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| 作者姓名： | 孙振元 郑湘如 徐楚年 林庆文 |
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| 基金项目： | 国家自然科学基金; 科委资助课题 |
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| 摘 要： | 本研究以棉花 BN-1(栽培种)和 MXZ-142(光籽突变种)为材料,通过对其受精和未受精胚珠的离体培养,明确了:受精 BN-1胚珠培养在 BT 培养基上能长出纤维,GA_3或GA_3和 IAA 能促进纤维伸长。而未受精 BN-1胚珠必需在 GA_3或 GA_3和 IAA 存在时才能长出纤维;MXZ-142胚珠无论受精与否均不能在 BT 培养基上正常生长,但当补充了 GA_3或 GA_3和IAA 后则生长良好;当有 GA_3存在于 BT-培养基中时,MXZ-142胚珠珠柄端和受伤部位均能产生绒状细胞群,它是由高度伸长的细胞组成的,来自受伤部位细胞或经过了愈伤组织阶段。认为这些细胞是早期发育阶段的纤维并具有发育为成熟纤维的能力。
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| 关 键 词： | 棉花 胚珠生长 纤维发育 离体培养 植物激素 |
The Study of Culturing Fibers from Derived Cells of Cotton Ovules |
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| Sun Zhenyuan Zheng Xiangru Xu Chunian Lin Qingwen.The Study of Culturing Fibers from Derived Cells of Cotton Ovules[J].Journal of China Agricultural University,1992(Z1):143-146. |
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| Authors: | Sun Zhenyuan Zheng Xiangru Xu Chunian Lin Qingwen |
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| Abstract: | The study indicated the effects of IAA and CA_s on ovule growth and fiber development in vitro.Beinong-1 caltivar (BN-1)and Xuzhou-142 fiberless mutant (MXZ-142) were used as experimental materials.Fertilized BN- 1 ovules Cultured in BT-medium produced fibers,and unfertilized ones produ- ced fibers only when IAA and/or GA:wereircluded in BT medium Fertilized and unfertilized MXZ-142 ovules cultured on BT-medium could neither produce fibers nor grew normally.When BT-medium included both IAA and GA_s,MXZ-142 ... |
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| Keywords: | cotton ovule growth fiber development culture in vitro plant hormone |
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