| 苹果原料中酵母菌的分离鉴定 |
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| 引用本文： | 王丽敏,李军,胡小松.苹果原料中酵母菌的分离鉴定[J].中国农业大学学报,2004,9(4):14-17. |
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| 作者姓名： | 王丽敏 李军 胡小松 |
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| 作者单位： | 中国农业大学,食品科学与营养工程学院,北京,100083 |
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| 基金项目： | 国家“十五”重大科技专项 ( 2 0 0 1BA5 0 1A2 1) |
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| 摘 要： | 利用酵母粉-葡萄糖琼脂培养基对15个批次苹果原料样本中的酵母菌进行分离，并用API 20C AUX菌种鉴定系统进行鉴定，得到的25株酵母菌分别属于假丝酵母菌属、酿酒酵母菌属和克勒克酵母菌属的6个种：克柔/平常假丝酵母菌(Candida krusei)13株，酿酒酵母菌(Saccharomyces cerevisiae)5株，克勒克酵母菌属酵母菌(Kloeckera spp.)2株，木蓝假丝酵母菌(C.magnoliae)2株，热带假丝酵母菌(C.tropicalis)和乳酒假丝酵母菌(C.kefyr)各1株，鉴定准确率均超过95％，其中模式菌酿酒酵母的鉴定准确率为98.7％，相似度1.00；鉴定评价均为“好”。该系统能够快速、准确地鉴定苹果原料中的酵母菌。
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| 关 键 词： | 苹果 酵母菌 API菌种鉴定系统 |
| 文章编号： | 1007-4333(2004)04-0014-04 |
| 修稿时间： | 2004年2月14日 |
Isolation and identification of yeasts from apple |
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| Wang Limin,Li Jun,Hu Xiaosong.Isolation and identification of yeasts from apple[J].Journal of China Agricultural University,2004,9(4):14-17. |
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| Authors: | Wang Limin Li Jun Hu Xiaosong |
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| Abstract: | A study was done to isolate and identify yeasts from apple, which (15 samples)were collected from the North Andre Apple Juice Co.Ltd. The yeasts were isolated with unpasteurized samples in the media of glucose yeast extract tryptone media. The identification of yeasts were done with the physiological characteristics and the API 20C AUX system. The Candida spp., Kloeckere spp. and Saccharomyces spp. were found in most samples. And high level of Candida spp. was detected in most samples. The 6 genera were Candida krusei (13), Saccharomyces cerevisiae (5), Kloeckera spp. (2), C. magnoliae (2), C. tropicalis (1)and C. kefyr (1). The accuracy of identification was >95 % . The API 20C AUX system could rapidly identify the clones of yeasts isolated from apple. |
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| Keywords: | apple yeasts API 20C AUX system |
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