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苹果AFLP分析体系的建立
引用本文:祝军,王涛,李光晨,赵玉军,张文,周爱琴.苹果AFLP分析体系的建立[J].中国农业大学学报,2000,5(3):63-67.
作者姓名:祝军  王涛  李光晨  赵玉军  张文  周爱琴
作者单位:1. 山东莱阳农学院园艺系,265200
2. 中国农业大学园艺学院
基金项目:国家自然科学基金 !39770 52 3,教育部博士点基金资助项目
摘    要:以12个苹果砧枯木基因型为试材,建立了扩增酶切片段长度多态性(AFLP)分析体系,对其分析过程中DNA提取、双酶切、连接、预扩增、标记和选择性扩增的效果进行了检测。用P32M64引物构建了供试苹果砧木基因型的AFLP指纹图谱。该指纹图谱拥有扩增条带105条,多态性带67条(占63.8%),条带清晰可辨,扩增信号强,无背景干扰。讨论了AFLP分析的影响因素。

关 键 词:AFLP分析  DNA指纹图谱  苹果
修稿时间:1999-09-15

Construction of AFLP Analysis System in Apple
Zhu Jun,Wang Tao,Li Guangchen,Zhao Yujun,Zhang Wen,Zhou Aiqin.Construction of AFLP Analysis System in Apple[J].Journal of China Agricultural University,2000,5(3):63-67.
Authors:Zhu Jun  Wang Tao  Li Guangchen  Zhao Yujun  Zhang Wen  Zhou Aiqin
Abstract:AFLP(Amplified Fragment Length Polymorphism)analysis system was established with 12 apple rootstock genotypes( Malus ). The results of several reactions during AFLP analysis have been detected such as DNA extraction,double enzymes restriction,adapter ligation,pre amplification,labeling and selective amplification. The AFLP fingerprintings of 12 apple rootstock genotypes with primer P32M64 have been performed,which had 105 clear and strong amplification bands with bright background. The factors which affect AFLP analysis were discussed.
Keywords:AFLP system  DNA fingerprinting  apple rootstocks
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