普通小麦农大3338×京冬6号DH系群体株高及节间长度的QTL分析

采用普通小麦农大3338和京冬6号的组合构建的包含216个株系的DH系为材料,以包含379个标记的高密度遗传连锁图谱为基础,利用复合区间作图法,通过一年两点田间试验,对株高及其组成成分不同节间长度的QTL进行分析。结果表明,一年两点最终株高共定位到8个QTL,分布在染色体2D,4B,4D,5A,6D,7A上,共解释株高变异为91.86%(北京)、92.63%(临汾)。各节间表型数据总共定位到28个QTL,分布在染色体2B,2D,3B,4A,4B,4D,5A,6A,6D,7A上。这些QTL基本包括了影响最终株高的8个位点,各节间长度还有部分特有的QTL。上述结果为在育种中实现对株高、穗下节长和其他节间长度的精细遗传操作及深入解析株高性状形成的遗传学基础提供了理论依据。

QTL mapping for plant height and internode lengths in Nongda3338×Jingdong6 DH populations of wheat(Triticum aestivum L.)

A double haploid line(DHL) population with 216 lines derived from a cross of Nongda 3338 and Jingdong 6 was used to identify the quantitative trait loci(QTL) for plant height and internode length in wheat(Triticum aestivum L.).Based on the high density genetic linkage map with 379 markers,eight QTLs were detected by composite interval mapping(LOD>2.5) using two locations phenotype data of plant height in the same year,and distributed on chromosomes 2D,4B,4D,5A,6D,7A respectively.The total QTL effects detected for the plant height accounted for 91.86%(Beijing)and 92.63%(Linfen)of the phenotypic variation in two locations.And 28 QTLs were detected using two locations phenotype data of five internodes length in the same year,and distributed on chromosomes 2B,2D,3B,4A,4B,4D,5A,6A,6D and 7A.Many QTLs for internode length are overlapping with the QTLs for plant height,indicating the high level of relevance between plant height and internode length.The present data are useful for wheat genetic manipulations through molecular marker-assisted selection(MAS),and provides new insights into understanding the genetic basis of plant height in wheat.