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| 京玉1号甜瓜高效再生体系的建立 | |
| 引用本文： | 胡莹,冷平,王福军,马斌,修莉莉.京玉1号甜瓜高效再生体系的建立[J].中国农业大学学报,2009,14(1). |
| 作者姓名： | 胡莹 冷平 王福军 马斌 修莉莉 |
| 作者单位： | 1. 中国农业大学,农学与生物技术学院,北京,100193 2. 中国农业大学,科学技术处,北京,100193 3. 北京市科学技术委员会,北京,100035 |
| 摘 要： | 以京玉1号甜瓜为材料,研究了外植体不同部位、苗龄、激素组合等因素对植株离体再生的影响,建立了以子叶为外植体的高效再生体系.结果表明:甜瓜子叶生长6 d时最佳,用Ms+6-BA 2.0 mg/L诱导分化培养基,不定芽诱导率可达88.67%;诱导不定芽伸长生长以MS+GA32 mg/L+KT 2 mg/L为最佳,伸长率达83.33%;伸长的不定芽在MS+IBA 0.5 mg/L上生根良好,外植体生根率达91.67%. |
| 关 键 词： | 京玉1号甜瓜 子叶 不定芽 再生体系 |
Establishment of efficient regeneration system for Cucumis melo L.cv.JingYu No.1 |
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| HU Ying,LENG Ping,WANG Fu-jun,MA Bin,XIU Li-li.Establishment of efficient regeneration system for Cucumis melo L.cv.JingYu No.1[J].Journal of China Agricultural University,2009,14(1). | |
| Authors: | HU Ying LENG Ping WANG Fu-jun MA Bin XIU Li-li |
| Institution: | 1.College of Agronomy and Biotechnology;China Agricultural University;Beijing 100193;China;2.Division of Science and Technology of China Agricultural University;3.Beijing Municipal Science and Technology Commission;Beijing 100035;China |
| Abstract: | To lay the foundation for applying transgenic technology to tissue culture and improve genetic characteristics of Cucumis melo L.cultivars,an efficient regeneration system,Cucumis melo L.cv.JingYu No.1 was developed with cotyledons as explants.We studied the effects of explants,seedling ages and combination of hormones on in vitro plant regeneration.The results showed that cotyledons excised from 6-day-old germfree seedlings were the best explants.The MS medium containing 6-BA 2.0 mg/L was suitable for indu... |
| Keywords: | Cucumis melo L cv JingYu No 1 cotyledons adventitious buds regeneration system |
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