板蓝根中靛蓝和靛玉红的提取及其质量分数的测定

采用直接比色法测定板蓝根中靛蓝、靛玉红的质量分数。对靛蓝、靛玉红在240～700nm波段进行扫描，确定其最大吸收波长分别为601和531nm。分别通过单因素试验和正交试验，确定提取靛蓝、靛玉红的最佳工艺参数。结果表明，乙醇体积分数为75％时，靛蓝和靛玉红的提取物得率最高；提取时间为4h时，靛蓝和靛玉红的提取物得率最高。正交试验确定的最佳提取工艺参数为：药材粒度40目，提取时间4h，乙醇体积分数75％，此时，提取物得率达1．74%。

Extraction and determination of mass ratio of Indigo and Indirubin in Indigowoad root

Extraction and determination of mass ratio of Indigo and Indirubin in Indigowoad root were studied. Indigo and Indirubin were scanned from 240 to 700*!nm. From the spectrogram we can see that the largest absorbing wavelength of Indigo and Indirubin was 601 and 531*!nm respectively. The best parameters of technology have been determined through single factor and orthogonal test on extracting indigo and Indirubin respectively, results showed that ethanol with a volume ratio of 75% was suitable solvent to extract Indigo and Indirubin and 4 hours was the proper time to extract Indigo and Indirubin. Through orthogonal test the best parameters of technology were obtained. The results are as the following: the herbal granularity is 40 eye, extraction time is 4*!h, ethanol with a volume ratio of 75%. On this condition, the extracting rate was up to 1.74%.