冬、春小麦幼穗离体培养的比较研究

本试验用三种类型的小麦幼穗为材料,在幼穗的发育时期,去分化和分化培养基等完全相同的条件下,对它们在离体培养下的反应进行了比较,发现愈伤组织的诱导率和苗的分化率以及愈伤组织在继代培养中的行为,都因不同类型的小麦幼穗而产生显著差异,而且愈伤组织的诱导率和苗的分化率之间不存在相关性。我们认为,材料的基因型选择,可能是建立小麦的胚性细胞悬浮培养物系统的一个有希望的途径。

A COMPARATIVE STUDY:SOME REACTIONS OF YOUNG PANICLES OF WINTER AND SPRING WHEAT CULTURED IN VITRO

The young panicles of winter and spring wheat cultured in the same differential or dedifferential condions(or media)in vitro.The results showed as follows(1)The percentages of callus induction and plantlet regeneration are different,depending on the genetypes of wheat;(2)Their calli represent various kinds of behaviour in subculture;(3)There is no relativity between the percentage of callus induction and plantlet regeneration;(4)we consider that the chosen genetypes may be a promising potential pathway for ...