小麦苗期浸种和根灌浅黄隐球酵母菌的定殖特征及对茎基腐病的防治效果

基于平板混合培养试验和浸种、根灌浅黄隐球酵母菌(Papiliotrema flavescens)温室盆栽试验,研究其在小麦苗期的定殖特征及对茎基腐病的防治效果。结果表明:1)在平板混合试验中,浅黄隐球酵母菌对禾谷镰刀菌的抑菌效果显著,抑菌率达84.1%;2)盆栽试验中,浅黄隐球酵母菌的定殖数量随时间推移呈减少趋势,浸种和灌根处理定殖数量均表现为叶鞘叶。与浅黄隐球酵母菌浸种处理相比,根灌浅黄隐球酵母菌处理的叶鞘浅黄隐球酵母菌定殖数量显著增加,并在接种后14d达到最大;3)盆栽试验中,根灌浅黄隐球酵母菌对禾谷镰刀菌的相对防效为22.7%,对假禾谷镰刀菌的相对防效为25.3%,均显著高于根灌生理盐水处理(P0.05),对小麦株高无影响。本研究表明通过根灌浅黄隐球酵母菌可以在小麦苗期实现定殖,并且对小麦苗期茎基腐病有一定防效。

Colonization characteristics of seed-soaking and root-irrigation with Papiliotrema flavescens in wheat seedling and the control of wheat crown rot

Based on the mixed plate cultivation and seed-soaking and root-irrigation Papiliotrema flavescens greenhouse pot experiments, the colonization characters of P. flavescens in wheat seedling and its inhibitory effects on crown rot were investigated. The main results are as follows: 1)P. flavescens showed a significant inhibition effect on the growth of Fusarium graminearum in the mixed plate cultivation test with an inhibition rate of 84. 1%; 2)In the pot experiment, the number of colonization of P. flavescens showed a decreasing trend over time, and the number of colonization in leaf sheath was more than that in leaf of both the seed-soaking and root-irrigation treatments. Furthermore, compared with the seed-soaking P. flavescens treatments, the number of colonization of P. flavescens in leaf sheath in the root-irrigation treatments was significantly higher in root-irrigation treatment, which reached the maximum colonization number at 14 d after inoculation; 3)In the pot experiment, the relative control effects of root-irrigation P. flavescens treatments on F. graminearum and F. pseudograminearum were 22. 7% and 25. 3%, respectively, both of which were significantly higher than that of the root-irrigation normal saline treatment(P<0. 05). Root-irrigation P. flavescens had no effect on the wheat height. In conclusion, this study displayed that the root irrigation of P. flavescens could achieve its colonization at wheat seedling period and exhibited control effect on wheat crown rot.