一株牦牛源产细菌素植物乳杆菌SWUN5815全基因组测序及序列分析

旨在分析一株牦牛源植物乳杆菌SWUN5815的全基因组序列测序,并对该菌株的功能特性基因进行挖掘。基于PacBio RSII测序平台对乳杆菌SWUN5815进行全基因组测序分析和GO、KEGG、COG和NR数据库进行基因组基本功能注释。结果表明:1)植物乳杆菌SWUN5815的基因组大小为3.27 Mb,GC含量44.59%;2)预测到3 258个编码基因,编码基因总长度为2 814 147bp,平均长度为864bp;3)编码基因通过功能数据库对该菌株基因组基本功能注释和代谢通路基因信息注释得知,SWUN5815基因组中包括5个基因参与抗氧化活性过程,2个基因参与免疫过程;基因组中包含了抗生素、生物降解等代谢过程;可调控免疫和炎症的相关通路;基因组中含有18种已知细菌素基因;有4个合成ABC型细菌素转运系统的功能序列。综上,SWUN5815全基因组测序及分析挖掘其特异性功能基因有利于研究其益生特性提供基础,为菌株作为抗生素替代品的益生菌和饲料添加剂的应用研究提供了重要的参考数据。

Whole-genome sequencing and sequence analysis of a Lactobacillus plantarum SWUN5815 producing bacteriocin from yak

The aim of this study was to analyze the whole genome sequence and its functional genes of Lactobacillus plantarum SWUN5815 from yak. Based on PacBio RSII sequencing platform, the whole genome of Lactobacillus SWUN5815 was sequenced and the basic functions of GO, KEGG, COG and NR databases were annotated. The results showed that: 1)The genome size of Lactobacillus plantarum SWUN5815 was 3. 27 Mb, and GC content was 44. 59%; 2)A total of 3 258 coding genes were predicted, with 2 814 147 bp length in total and an average length of 864 bp; 3)According to annotation of the basic functions and metabolic pathway genes on the genome of SWUN5815 through the functional database, there were five genes involved in the antioxidant activity process and two genes involved in immune process in the genome of SWUN5815. The genome contained metabolic processes such as antibiotics and biodegradation. The pathways that can regulate immunity and inflammation were related. There are 18 known genes in the genome and 4 functional sequences of ABC type bacteriocin transport system. In conclusion, the whole genome sequencing of SWUN5815 and the analysis of its specific functional genes were helpful to study its probiotic characteristics, which provided important reference for probiotics as antibiotic substitutes and feed additives.