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羊催乳素的纯化、理化鉴定及抗血清的制备
引用本文:夏国良,郭亚平,杨传任.羊催乳素的纯化、理化鉴定及抗血清的制备[J].中国农业大学学报,1989(3):330-335.
作者姓名:夏国良  郭亚平  杨传任
摘    要:本试验对绵羊垂体前叶催乳素进行了提纯和生物学、生物化学及免疫学方面的鉴定.516g 羊全垂体经硫酸铵、乙醇等的逐级提取,最后经 DEAE-纤维素柱层析得到催乳素纯品450mg.用鸽嗉囊局部皮内微量注射法测得其生物学活性为27国际单位(27IU/mg)。PAG 电泳表明有三条带。SDS 电泳测得其分子量为23000。IEFP 电泳表明平均等电点为5.85。用 Dansyl-Cl 法测定其 N-末端氨基酸,只显出苏氨酸一点。表明提纯的催乳素是高纯的。用该催乳素免疫家兔,九周后用琼脂双扩散法测抗血清,其效价最高达1:32,说明该纯化品具有很强的免疫原件.

关 键 词:羊催乳素  提纯  鉴定  抗血清  

ISOLATION AND CHARACTERIZATION OF OVINE PITUITARY PROLACTIN AND PRODUCTION OF ITS ANTISERUM
Xia Guoliang Guo Yaping Yang Chuanren.ISOLATION AND CHARACTERIZATION OF OVINE PITUITARY PROLACTIN AND PRODUCTION OF ITS ANTISERUM[J].Journal of China Agricultural University,1989(3):330-335.
Authors:Xia Guoliang Guo Yaping Yang Chuanren
Abstract:Through a series of extraction and precipitation with ammonium sulfate and chro- matography on DEAE-cellulose,we achieved 450rag purified prolactin from 516g sheep whole frozen pituitary.The yield was 0.87g per kilogram frozen pituitary. With the method of local micro-introdermal injection over the pigeon crop sac, we deduced that our sample possessed an activity of 27IU/per rag.On the polycry- lamide gel electrophoresis,the sample ran into three distinct bands.The SDS- PAG electrophoresis showed that the m...
Keywords:sheep prolactin  isolation  characterization  antisera  
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