红麻细胞质雄性不育系与保持系苗期耐冷生理研究

以7组红麻细胞质雄性不育系/保持系为材料,研究在12℃/6℃(昼/夜)低温胁迫下幼苗叶片相对电导率、丙二醛、脯氨酸、可溶性糖、可溶性蛋白含量及SOD和POD活性的变化,结果表明:持续低温胁迫下,红麻叶片的相对电导率、脯氨酸、可溶性糖、可溶性蛋白含量变化和SOD活性表现为先升高后降低;而POD活性和丙二醛含量则在胁迫9～15d后增加。利用隶属函数法和干物质重2种方法评定红麻材料间耐寒性强弱,发现相对电导率、可溶性蛋白和可溶性糖是反映红麻耐冷性差异的基本指标,上述2种方法比较红麻耐寒性强弱,其相关性达0.837**。供试的材料组合中耐寒性较强的材料分别是917A/B、722A/B和K03A/B。本研究结果可为今后进一步研究红麻耐冷防御措施和低温生理生化育种提供理论参考。

Physiological research of cold resistance on CMS and maintainer lines in kenaf leaves

Seven groups of cytoplasmic male sterile lines(CMS)/ maintainer lines of kenaf were used to study the changes of relative electric conductivity(Rec),contents of MDA、proline,soluble sugar,soluble protein and the activities of SOD and POD under low temperature(12 ℃/6 ℃,day/night).Results showed that the changes of Rec,contents of proline,soluble sugar,soluble protein and the activities of SOD were increased initially then decreased.However,the changes of MDA and the activity of POD were increased significantly after 9-15 days low temperature stress.The Rec,soluble sugar and soluble protein were the basic indicators in evaluating the difference in cold stress of kenaf,and the correlation of the two methods of evaluation on subordinate function value and dry matter weight was 0.837(P<0.01);The three groups of 917A/B,722A/B,K03A/B were performed very well under cold stress condition.The results provided theoretical foundation for further developing cold damage protection methods and breeding for low temperature tolerance.