| 投喂蚕豆对草鱼免疫器官超微结构和免疫基因表达的影响 |
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| 引用本文： | 陈论践,郁二蒙,王广军,谢骏,余德光,李志斐,张凯,龚望宝,田晶晶.投喂蚕豆对草鱼免疫器官超微结构和免疫基因表达的影响[J].中国农业大学学报,2019,24(6):92-103. |
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| 作者姓名： | 陈论践 郁二蒙 王广军 谢骏 余德光 李志斐 张凯 龚望宝 田晶晶 |
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| 作者单位： | 中国水产科学研究院珠江水产研究所;上海海洋大学水产与生命学院 |
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| 基金项目： | 现代农业产业技术体系建设专项（CARS-45-21）；中国水产科学研究院基本科研业务费资助项目（2017HY-ZC0501） |
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| 摘 要： | 为研究草鱼摄食蚕豆后免疫功能变化及转投30 d人工饲料后免疫增强效果,在草鱼摄食蚕豆30、60、90、120及150 d(转投饲料30 d)时,检测肠道、头肾、肝脏等器官超微结构及C-type lysozyme、IFN-I、TNF-α等免疫基因表达变化。结果表明:1)饲喂单一饲料蚕豆的草鱼,其肠道、头肾、肝脏等3个重要免疫器官受到严重损伤,转投饲料30 d后其病变并未显著好转;2)免疫基因C-type lysozyme、IFN-I、TNF-α、IgM和MHC-I的表达在30～120 d均被抑制,转投饲料30 d后,头肾中C-type lysozyme、IL-1β、IFN-I、IgM、MHC-I以及肝脏中IL-1β、TNF-α、IFN-I、IgM、MHC-I的表达量有所增加。综上,草鱼摄食蚕豆后其免疫功能受到显著抑制,而转投30 d饲料对脆肉鲩的免疫机能具有一定的恢复作用。
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| 关 键 词： | 草鱼 蚕豆 免疫器官 超微结构 免疫基因表达 |
| 收稿时间： | 2018/9/24 0:00:00 |
Effects of feeding with broad bean on the immune organ ultrastructure and expression of immune genes of grass carp |
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| CHEN Lunjian,YU Ermeng,WANG Guangjun,XIE Jun,YU Deguang,LI Zhifei,ZHANG Kai,GONG Wangbao and TIAN Jingjing.Effects of feeding with broad bean on the immune organ ultrastructure and expression of immune genes of grass carp[J].Journal of China Agricultural University,2019,24(6):92-103. |
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| Authors: | CHEN Lunjian YU Ermeng WANG Guangjun XIE Jun YU Deguang LI Zhifei ZHANG Kai GONG Wangbao and TIAN Jingjing |
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| Institution: | Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China;College of Fisheries and Life, Shanghai Ocean University, Shanghai 201306, China,1. Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China,Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China,Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China,Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China,Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China,Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China,Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China and Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China |
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| Abstract: | |
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| Keywords: | grass carp broad bean immune organ ultrastructure expression of immune gene |
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