| 利用SLAF-seq技术开发十倍体长穗偃麦草专化分子标记 |
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| 引用本文： | 杨国堂,罗巧玲,刘利勤,张静,李振声,郑琪.利用SLAF-seq技术开发十倍体长穗偃麦草专化分子标记[J].中国农业大学学报,2019,24(1):1-5. |
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| 作者姓名： | 杨国堂 罗巧玲 刘利勤 张静 李振声 郑琪 |
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| 作者单位： | 中国科学院遗传与发育生物学研究所/植物细胞与染色体工程国家重点实验室;中国科学院大学 |
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| 基金项目： | 国家重点研发计划项目资助（2016YFD0102000） |
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| 摘 要： | 为开发长穗偃麦草(Thinopyrum ponticum)分子标记,以小偃68(西农)为试验材料,利用SLAF-seq技术获得9 667个小偃68外源染色体特异标签,筛选出823个长穗偃麦草专化分子标记。选取其中100个标记扩增20份小偃麦易位系,发现染色体构成相似的易位系具有相同的扩增结果,并得到小偃麦易位系的特异分子标记。应用这些标记可以快速检测长穗偃麦草遗传物质。
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| 关 键 词： | 长穗偃麦草 远缘杂交 SLAF-seq 分子标记 |
| 收稿时间： | 2018/2/9 0:00:00 |
Development of Thinopyrum ponticum specific molecular markers by using SLAF-seq technology |
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| YANG Guotang,LUO Qiaoling,LIU Liqin,ZHANG Jing,LI Zhensheng and ZHENG Qi.Development of Thinopyrum ponticum specific molecular markers by using SLAF-seq technology[J].Journal of China Agricultural University,2019,24(1):1-5. |
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| Authors: | YANG Guotang LUO Qiaoling LIU Liqin ZHANG Jing LI Zhensheng and ZHENG Qi |
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| Institution: | State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Science, Beijing 100101, China;University of Chinese Academy of Sciences, Beijing 100049, China,State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Science, Beijing 100101, China;University of Chinese Academy of Sciences, Beijing 100049, China,State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Science, Beijing 100101, China,State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Science, Beijing 100101, China,State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Science, Beijing 100101, China and State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Science, Beijing 100101, China |
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| Abstract: | In order to develop markers of Thinopyrum ponticum, Xiaoyan 68 (XN) was taken as study material. A total of 9 667 specific fragments from the alien chromosomes of Xiaoyan 68 (XN) were screened by specific-locus amplified fragment sequencing (SLAF-seq) technology and 823 alien chromosome specific markers were developed. The results of PCR amplification analysis and the chromosome composition in wheat-Thinopyrum translocations showed that the lines amplified the same molecular markers carried the similar exogenous chromosome fragments. Specific markers of wheat-Thinopyrum translocations were obtained. All these newly developed specific markers could be used for rapid detection of the exogenous chromosome fragments in wheat-Th. ponticum progenies. |
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| Keywords: | Thinopyrum ponticum wide hybridization SLAF-seq molecular markers |
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