| 水产动物源气单胞菌喹诺酮类耐药与PMQR基因、QRDR突变相关性分析 |
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| 引用本文： | 谭爱萍,邓玉婷,姜兰,赵飞,张瑞泉.水产动物源气单胞菌喹诺酮类耐药与PMQR基因、QRDR突变相关性分析[J].中国农业大学学报,2019,24(4):102-111. |
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| 作者姓名： | 谭爱萍 邓玉婷 姜兰 赵飞 张瑞泉 |
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| 作者单位： | 中国水产科学研究院珠江水产研究所/农业部渔用药物创制重点实验室/广东省水产动物免疫技术重点实验室 |
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| 基金项目： | 广东省自然科学基金项目（2015A030313701）；现代农业产业技术体系专项（CARS-46）；中国水产科学院基本科研业务费资助项目（2017HY-ZD1004）；广东省渔港建设和渔业产生发展专项（A201601B07） |
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| 摘 要： | 为了解PMQR基因、QRDR突变与气单胞菌喹诺酮类耐药相关性,以116株水产源气单胞菌为研究对象,采用PCR法检测PMQR基因(qnrA、qnrB、qnrS、qepA、aac(6′)-Ib-cr)并分析QRDR靶基因gyrA、parC突变情况,用微量二倍稀释法测定萘啶酸(NAL)、环丙沙星(CIP)、恩诺沙星(ENR)的最小抑菌浓度(MIC)值。结果表明:116株气单胞菌中,有18株(15.52%)菌株携带PMQR基因,其中8株(6.90%)携带qnrS2基因,9株(7.76%)携带aac(6′)-Ib-cr基因,1株同时携带qnrS2、aac(6′)-Ib-cr基因,未检测到qnrA,qnrB和qepA基因;56株(48.28%)菌株发生QRDR靶位点突变,主要突变方式为GyrA:Ser~(83)Ile和GyrA:Ser~(83)Ile+ParC:Ser~(87)Ile;对NAL、CIP及ENR耐药率分别为47.41%、12.07%及11.21%;7株仅携带qnrS2基因菌株未发生QRDR突变,对喹诺酮类药物敏感性略下降,NAL、CIP及ENR的MIC值分别小于4.00、0.50和1.00mg/L;10株携带aac(6′)-Ib-cr基因的菌株均发生QRDR突变并对NAL表现耐药,MIC值均128.00mg/L,其中8株对CIP和ENR表现耐药,MIC值均≥4.00mg/L;发生gyrA单突变或gyrA和parC双突变菌株均对NAL表现耐药,MIC值均≥64.00mg/L,CIP和ENR的MIC值有的4.00mg/L,有的≥4.00mg/L。综上,QRDR靶基因突变可影响水产动物源气单胞菌对萘啶酸的药物敏感性,而气单胞菌对氟喹诺酮类药物耐药可能与PMQR和QRDR协同作用有关。
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| 关 键 词： | 气单胞菌 PMQR基因 QRDR突变 喹诺酮类 耐药 |
| 收稿时间： | 2018/6/14 0:00:00 |
Correlation between PMQR genes/QRDR mutations and quinolones resistance in Aeromonas from aquatic animals |
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| TAN Aiping,DENG Yuting,JIANG Lan,ZHAO Fei and ZHANG Ruiquan.Correlation between PMQR genes/QRDR mutations and quinolones resistance in Aeromonas from aquatic animals[J].Journal of China Agricultural University,2019,24(4):102-111. |
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| Authors: | TAN Aiping DENG Yuting JIANG Lan ZHAO Fei and ZHANG Ruiquan |
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| Institution: | Pearl River Fisheries Research Institute/Key Laboratory of Fishery Drug Development of Ministry of Agriculture/Key Laboratory of Aquatic Animal Immune Technology of Guangdong Province, Chinese Academy of Fishery Sciences, Guangzhou 510380, China,Pearl River Fisheries Research Institute/Key Laboratory of Fishery Drug Development of Ministry of Agriculture/Key Laboratory of Aquatic Animal Immune Technology of Guangdong Province, Chinese Academy of Fishery Sciences, Guangzhou 510380, China,Pearl River Fisheries Research Institute/Key Laboratory of Fishery Drug Development of Ministry of Agriculture/Key Laboratory of Aquatic Animal Immune Technology of Guangdong Province, Chinese Academy of Fishery Sciences, Guangzhou 510380, China,Pearl River Fisheries Research Institute/Key Laboratory of Fishery Drug Development of Ministry of Agriculture/Key Laboratory of Aquatic Animal Immune Technology of Guangdong Province, Chinese Academy of Fishery Sciences, Guangzhou 510380, China and Pearl River Fisheries Research Institute/Key Laboratory of Fishery Drug Development of Ministry of Agriculture/Key Laboratory of Aquatic Animal Immune Technology of Guangdong Province, Chinese Academy of Fishery Sciences, Guangzhou 510380, China |
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| Abstract: | |
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| Keywords: | Aeromonas PMQR QRDR mutation quinolones resistance |
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