树干注药对柳树几种保护酶活性及游离脯氨酸含量的影响

以垂柳为材料研究4种注干药剂(吡虫啉、啶虫脒、吡虫啉.敌敌畏和敌畏.氧乐)树干注药后其叶片内几种保护酶活性及游离脯氨酸含量的变化。结果表明:4种药剂树干注药后垂柳叶片内超氧化物歧化酶(SOD)活性均呈现上升-下降-上升的过程。敌畏.氧乐对SOD影响最明显,药后1 d活性上升了85.01%,6 d活性下降了35.99%,20 d上升了32.00%。各药剂处理均可提高多酚氧化酶(PPO)和苯丙氨酸解氨酶(PAL)的活性,抑制过氧化物酶(POD)活性。吡虫啉对PPO刺激作用最明显,药后10 d活性上升了21.87%;对POD抑制作用最显著,药后6 d活性下降了24.78%。敌畏.氧乐对PAL活性影响最显著,药后6 d活性上升了28.97%。各药剂处理均可提高游离脯氨酸的含量,其中敌畏.氧乐的影响最显著,药后3 d含量增加了52.91%。树干注药30 d后各处理组SOD、POD、PPO和PAL活性及游离脯氨酸的含量均逐渐恢复到对照水平。

Pesticide effects on some protective enzymes and dissociative proline in willow by trunk injection

The effects of four types of liquid pesticides(imidacloprid,acetamiprid,imidacloprid DDVP and DDVP omethoate) on some protective enzymes and dissociative proline in weeping willow(Salix babylonica Linn) leaves through trunk injection were reported.The result showed that the activity of SOD experienced a raising-descending-raising process after a trunk injection.Among four pesticides,DDVP omethoate affected the activity of SOD greatly,the activity was increased 85.01% within one day,reduced 35.99% within six days and increased 32.00% within twenty days after a trunk injection.All of four pesticides caused the increase of PPO and PAL activity and the decrease of POD activity.Imidacloprid seriously affected PPO and POD activities,the activity of PPO was increased 21.87% within ten days after a trunk injection,and the activity of POD was reduced 24.78% within six days after a trunk injection.DDVP omethoate affected PAL activity greatly;the activity was increased 28.97% within six days after a trunk injection.The content of dissociative proline had also descended after treated by the four pesticides through trunk injection.DDVP omethoate affected the content of proline greatly;the content was increased 52.91% within three days after a trunk injection.The activity of SOD,POD,PPO,PAL and the content of dissociative proline had come back to the control level within thirty days after a trunk injection.