组织切片法观察昆明白小鼠卵丘扩展与卵母细胞成熟的关系

三实验应用组织切片法探讨了体内正常生理条件下昆明白小鼠卵巢内卵丘扩展与卵母细胞成熟的关系。初情期前小鼠注射孕马血清促性腺激素，４８ｈ后注射人绒毛膜促性腺激素，于注射ＨＣＧ后不同时间取出卵巢进行组织切片。注射ＨＣＧ后１ｈ，卵丘细胞开始扩展，此时卵母细胞核膜完整，即未发生生发泡破裂；注射ＨＣＧ后３ｈ，大部分大有腔卵泡中卵母细胞发生ＧＶＢＤ，此时卵丘处于２级扩展状态。卵丘完全扩展发生在注射ＨＣＧ后９ｈ，

Histologic Study of the Relationship between Cumulus Expansion and Oocyte Maturation in the Kunming White Mouse

The timing of cumulus cell expansion and oocyte maturation was investigated in vivo , using H and E stained paraffin sections of ovary isolated from immature Kunming white mice treated with gonadotropins. One hour after the injection of an ovulatory dose of HCG, expansion of cumulus cells in the outer layer of large antral follicles was observed, but oocyte maturation as evidenced by germinal vesicle breakdown (GVBD) was not evident. Three hours after HCG injection, the majority of oocytes in the large antral follicle (70%) had undergone GVBD, but the cumulus cells were not completely expanded at this time. Cumulus expansion appeared to be completed by 9hr after HCG injection, however, oocyte maturation occurred about 3hr later than cumulus expansion. The results indicate that primary cumulus expansion occurred prior to oocyte maturation, whereas cumulus mass complete expansion is not necessary for maturation of oocyte in vivo.