生长猪胃肠道乳酸菌、双歧杆菌和大肠杆菌的数量和分布规律

本研究采用严格的厌氧微生物培养方法，对5头生长猪进行了测定。结果表明，在生长猪的胃肠道内容物中和粘膜上均有相当数量的乳酸菌、双歧杆菌和大肠杆菌分布，但乳酸菌的数量显著高于其他2种菌（P＜0．01）。3类菌在内容物中的分布为：乳酸菌在猪后肠内容物中的数量分布显著高于胃和小肠（P＜0．01）；双歧杆菌在盲肠内容物中数量最高（P＜0．01）；而盲肠和结构内容物中大肠杆菌的数量分布大于其他部位（P＜0．01）。粘膜的分布为：乳酸菌在结肠，双歧杆菌和大肠杆菌在盲肠粘膜上的数量分布显著高于其他各区段（P＜0．05）。猪胃肠道各区段中，除直肠内容物中双歧杆菌的数量显著高于大肠杆菌外（P＜0．01），其他各区段中2种菌的数量统计差异不显著（P＞0．05）；结肠和直肠粘膜上双歧杆菌的数量显著高于大肠杆菌（P＜0．05）。

Numeration of Lactic Acid Producing Bacteria, Bifidobacteria and Escherichia coli Populations in GastrointestinalTract of Growing Pig

A study was conducted to determine the number of lactic acid Producing bacteria, Bifidobacteria and Escherichia coli in the gastrointestinal contents and associated with mucus layers of various segments (gastric, small intestine, cecum, colon and rectum) of growing pigs. Results showed that there were significant bacterial populations presenting in gastrointestinal contents and mucus layers along the whole gastrointestinal tract. Furthermore, the population of lactic acid Producing bacteria presented both in gastrointestinal contents and associated with mucus layers was significantly higher than that of Bifidobacteria or E. coli ( P <0.01). The distribution of the three groups in the contents of the whole tract was: the number of Lactic acid Producing bacteria in the hindgut contents was significantly higher than that of gastric or small intestine ( P <0.01); the populations of Bifidobacteria in the cecum contents was the greatest ( P <0.01); the concentration of E. coli in the contents of cecum and colon was higher than that of other segments ( P <0 01). The distribution of the three groups associated with the gastrointestinal mucus layers was: the populations of Lactic acid producing bacteria in colon, Bifidobacteria or E. coli in cecum and colon were significantly greater than that of other segments of gastrointestinal tract ( P <0.05). There was no significant difference of the populations between Bifidobacteria and E. coli in the gastrointestinal contents except in the rectum. The numbers of Bifidobacteria associated with the colon and rectum mucus layers were significantly higher than that of E. coli ( P <0.05).