| 月季“黄和平”体细胞胚诱导的初步研究 |
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| 引用本文： | 尤扬,金典生.月季“黄和平”体细胞胚诱导的初步研究[J].湖北农业科学,2012,51(10):2128-2131. |
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| 作者姓名： | 尤扬 金典生 |
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| 作者单位： | 1. 河南科技学院园艺园林学院,河南新乡,453003
2. 河南机电高等专科学校,河南新乡,453000 |
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| 基金项目： | 河南科技学院重点科研基金资助项目(050122) |
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| 摘 要： | 以月季(Rosa chinensis)“黄和平”的幼嫩叶片为外植体,研究了不同植物生长调节剂对其愈伤组织和体细胞胚诱导的影响.结果表明,在愈伤组织诱导试验中,MS+3 mg/L 2,4-D+0.5 mg/L 6-BA效果最好,诱导率达94.8％;在体细胞胚诱导试验中,其最适诱导培养基为MS+1.2 mg/L TDZ,暗培养8d后转入光下培养,体细胞胚诱导率最高,达33.3％,暗培养超过8d,外植体易形成愈伤组织而不利于体细胞胚的诱导.
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| 关 键 词： | 月季(Rosa chinensis) 叶片 愈伤组织 体细胞胚 |
Prelimitary Study on Somatic Embryo Induction in Rosa chinensis |
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| YOU Yang
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JIN Dian-sheng.Prelimitary Study on Somatic Embryo Induction in Rosa chinensis[J].Hubei Agricultural Sciences,2012,51(10):2128-2131. |
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| Authors: | YOU Yang JIN Dian-sheng |
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| Institution: | 1.School of Horticulture and Landscape Architecture,Henan Institute of Science and Technology,Xinxiang 453003,Henan,China; 2.Henan Mechanical and Electrical Engineering College,Xinxiang 453000,Henan,China) |
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| Abstract: | Using tender leaves of Rosa chinensis cv.’Yellow Peace’ as explants,the effects of different growth regulator on tissue culture and somatic embryogenesis induction were studied.The results showed that MS+3 mg/L 2,4-D+0.5 mg/L 6-BA was the best for callus induction,with an induction rate of 94.8%.In the somatic embryogenesis induction test,dark induction for 8 d in MS+1.2 mg/L TDZ before light culture,the regeneration rate was the highest,with an induction rate of 33.3%.If dark culture time was longer than 8 d,callus would easily formed while the regeneration frequency could be reduced. |
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| Keywords: | Chinese rose(Rosa chinensis) leaf callus somatic embryo |
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