| 一株抗铬芽孢杆菌的分离鉴定及16S rDNA序列分析 |
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| 引用本文： | 龚汉雨,程国军.一株抗铬芽孢杆菌的分离鉴定及16S rDNA序列分析[J].湖北农业科学,2009,48(6). |
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| 作者姓名： | 龚汉雨 程国军 |
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| 作者单位： | 中南民族大学生命科学学院,武汉,430074 |
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| 基金项目： | 中南民族大学自然科学基金重点项目 |
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| 摘 要： | 从湖北省大冶市某矿区土壤中分离到1株可以在含7mmo·L-1铬(Cr6+)的培养基上正常生长的菌株,命名为MD08.对该菌株进行了形态学观察和生理生化鉴定以及16S rDNA序列分析.结果表明,MD08为革兰氏阳性细菌,椭圆形,中央有1个大的芽孢,利用葡萄糖产气产酸,甲基红试验显示为阴性;通过对该菌株的16S rDNA进行测序和同源性比较,发现它与芽孢杆菌的同源性达到99%,该菌株被鉴定为巨大芽孢杆菌(Bacillus megaterium);MD08对多种重金属普遍具有较高的抗性,可以用于重金属污染的微生物修复和研究微生物与重金属的相互作用.
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| 关 键 词： | 抗铬微生物 芽孢杆菌 |
Isolation, Identification and 16S rDNA Sequences Analysis of A Cr(Ⅵ)-Resisting Bacterium |
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| GONG Han-yu,CHENG Guo-jun.Isolation, Identification and 16S rDNA Sequences Analysis of A Cr(Ⅵ)-Resisting Bacterium[J].Hubei Agricultural Sciences,2009,48(6). |
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| Authors: | GONG Han-yu CHENG Guo-jun |
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| Institution: | College of Life Sciences;Zhongnan University for Nationalities;Wuhan 430074;China |
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| Abstract: | A bacterial strain MD08,which was able to survive to 7 mmol.L-1 Cr(VI),was isolated from the soils in mineral area of Daye city,Hubei province.A series observertions of morphological and biochemical characteristics and the analysis of 16S rDNA sequence revealed that MD08 was gram-positive,oval-shaped bacteria,and glucose can be utilited to produce gas and acid,methyl-red test displayed negative.In addition,MD08 had 99% homologous to Bacillus by 16S rDNA se-quence analysis.It was identified as Bacillus megat... |
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| Keywords: | 16S rDNA |
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