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| 不同基本培养基和激素对籼稻成熟胚愈伤组织诱导的影响 | |
| 引用本文： | 廖鹏飞,赵君幸,雷发扬,余潮,李绍波.不同基本培养基和激素对籼稻成熟胚愈伤组织诱导的影响[J].湖北农业科学,2012,51(18):4121-4125. |
| 作者姓名： | 廖鹏飞 赵君幸 雷发扬 余潮 李绍波 |
| 作者单位： | 1. 南昌大学生命科学与食品工程学院/南昌大学生物学实验教学中心,南昌 3300313;南昌大学江西省分子生物学与基因工程重点实验室,南昌330031 2. 南昌大学生命科学与食品工程学院/南昌大学生物学实验教学中心,南昌,3300313 3. 南昌大学江西省分子生物学与基因工程重点实验室,南昌,330031 |
| 基金项目： | 国家自然科学基金项目(30860155);江西省教育厅科技项目(GJJ12013) |
| 摘 要： | 以川香29B、中9B、金23B和Ⅱ-32B 4种籼稻的成熟胚为外植体,研究MS、N6、NB和NMB 4种基本培养基,2,4-D与KT或6-BA联合使用对籼稻愈伤组织诱导的影响.结果表明,4种籼稻在NMB基本培养基中愈伤组织的诱导率最高,平均诱导率为77.00％～96.00％,其中在NMB中的诱导效果最好,在4种不同基本培养基中愈伤组织总平均诱导率的高低顺序依次为川香29B、中9B、Ⅱ-32B、金23B.当3.0mg/L 2,4-D与不同浓度的KT或6-BA联合使用对,KT浓度为0.1 mg/L对,川香29B和中9B愈伤组织诱导率高；对于Ⅱ-32B,KT浓度为0.6 mg/L愈伤组织诱导率较高；6-BA浓度为0.4 mg/L时,川香29B愈伤组织诱导率最高；对于中9B和Ⅱ-32B,添加0.1 mg/L的6-BA最有利于愈伤组织的诱导；而对于金23B,无论是添加KT或6-BA都会抑制愈伤组织的诱导. |
| 关 键 词： | 籼稻 愈伤组织诱导 培养基 激素 |
Effects of Different Basal Medium and Phytokinin on Callus Induction of Indica Rice Mature Embryos |
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| LIAO Peng-fei , ZHAO Jun-xing , LEI Fa-yang , YU Chao , LI Shao-bo.Effects of Different Basal Medium and Phytokinin on Callus Induction of Indica Rice Mature Embryos[J].Hubei Agricultural Sciences,2012,51(18):4121-4125. | |
| Authors: | LIAO Peng-fei ZHAO Jun-xing LEI Fa-yang YU Chao LI Shao-bo |
| Institution: | 1.Teaching Center for Experimental Biology / School of Life Science and Food Engineering,Nanchang University,Nanchang 330031,China;2.Key Laboratory of Molecular Biology and Gene Engineering in Jiangxi Province,Nanchang University,Nanchang 330031,China) |
| Abstract: | Effects of four basal medium(MS,N6,NB and NMB) and two kinds of phytokinin(KT,6-BA) on callus induction from mature embryos of four indica rice varieties(Chuanxiang29B,Zhong9B,Jin23B,Ⅱ-32B) were investigated.The results showed that the most efficient basal medium was NMB as the average callus induction rates ranged from 77.00% to 96.00%.The order of average rate from high to low was Chuanxiang29B,Zhong9B,Ⅱ-32B,Jin23B.When 3 mg/L 2,4-D was applied with 0.1 mg/L KT or 0.4 mg/L 6-BA,the induction rate of Chuangxiang29B was the highest.For Zhong9B,the combination of 3 mg/L 2,4-D with 0.1 mg/L KT or 0.1 mg/L 6-BA was optimum.ForⅡ-32B,the combination of 3 mg/L 2,4-D with 0.6 mg/L KT or 0.1 mg/L 6-BA was optimum.However,the callus formation of Jin23B would be inhibited by adding either 6-BA or KT. |
| Keywords: | indica rice callus induction basal medium phytokinin |
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