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普通百里香的组织培养和快速繁殖
引用本文:李晓东,王永飞,马三梅,程智慧,邓盾.普通百里香的组织培养和快速繁殖[J].湖北农业科学,2009,48(6).
作者姓名:李晓东  王永飞  马三梅  程智慧  邓盾
作者单位:1. 深圳职业技术学院,广东,深圳,518055
2. 暨南大学生物工程学系,广州,510632
3. 西北农林科技大学园艺学院,陕西,杨凌,712100
4. 深圳职业技术学院,广东,深圳,518055;暨南大学生物工程学系,广州,510632
基金项目:深圳职业技术学院重点科研项目 
摘    要:试验建立了一种普通百里香的快速繁殖方法.以带腋芽的茎段为外植体,比较了不同浓度的IAA和6-BA组合对不定芽的诱导和增殖的效果,以及不同浓度的IBA和多效唑组合对枝条生根的影响.结果表明.不定芽的增殖和诱导以MS+IAA 0.25 mg·L-1+6-BA 0.25 mg·L-1为宜,生根以1/2MS+IBA0.50mg·L-1+PP333 0.75 mg·L-1为宜.

关 键 词:百里香  快速繁殖  茎段

Tissue Culture and Rapid Propagation of Thymus vulgaris
LI Xiao-dong,WANG Yong-fei,MA San-mei,CHENG Zhi-hui,DENG Dun.Tissue Culture and Rapid Propagation of Thymus vulgaris[J].Hubei Agricultural Sciences,2009,48(6).
Authors:LI Xiao-dong  WANG Yong-fei  MA San-mei  CHENG Zhi-hui  DENG Dun
Institution:1.Department of Applied Biological Engineering;Shenzhen Polytechnic;Shenzhen 518055;Guangdong;China 2.Department of Biotechnology;Jinan University;Guangzhou 510632;China;3.Academy of Horticulture;Northwestern Sci-Technology of Agricultural and Forestry University;Yangling 712100;Shaanxi;China
Abstract:A rapid propagation method of Thymus vulgaris was established.The study compared the effect of various IAA and 6-BA on stem with axillary bud of Thymus vulgaris for inducing and propagating adventitious buds,and compared the effect of various concentration of IBA and paclobutarzol on shoots for inducing roots.The results showed that MS+IAA 0.25 mg.L-1+ 6-BA 0.25 mg.L-1 was suitable for inducing and propagating of adventitious buds,and 1 / 2MS+IBA 0.50 mg.L-1+PP333 0.75 mg.L-1 for generating roots.
Keywords:Thymus vulgaris  rapid propagation  stem segments  
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