| 枯萎病菌诱导的节瓜抑制差减cDNA文库的构建 |
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| 引用本文： | 曾永三,孙辉,黎志轩,刘开启.枯萎病菌诱导的节瓜抑制差减cDNA文库的构建[J].湖北农业科学,2010,49(3). |
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| 作者姓名： | 曾永三 孙辉 黎志轩 刘开启 |
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| 作者单位： | 仲恺农业工程学院植物保护系,广州,510225 |
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| 基金项目： | 广东省自然科学博士启动基金，广东省植物保护新技术重点实验室开放基金 |
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| 摘 要： | 以抗枯萎病节瓜品种杂交20号为材料,利用抑制差减杂交(Suppression subtractive hybridization,SSH)技术,构建了枯萎菌(Fusarium oxysporum f.sp.beniacasa)诱导的节瓜SSH-cDNA文库。文库质量检测表明,差减杂交效率较高,质量较好。得到正向差减文库的滴度为3.64×105 CFU/mL,反向差减文库滴度为6.80×105 CFU/mL;正向和反向差减文库的重组率均大于90%,插入片段在400～800bp之间。
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| 关 键 词： | 节瓜枯萎病 抑制差减杂交 cDNA文库 |
Construction of Chieh-qua Suppression Subtractive Hybridization cDNA Library Induced by Fusarium oxysporum f. sp. beniacasa |
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| Abstract: | By a suppression subtractive hybridization technique, a suppression subtractive hybridization cDNA library was constructed with resistant Chieh-qua cultivar Zajiao 20 leaf induced by Fusarium oxysporum f. sp. beniacasa. Detection and analysis of the library construction showed that the technique was efficient in the subtractive hybridization and a good qualitySSH-cDNA library was obtained. The titers of forward-and reverse-SSH-cDNA libraries were 3.64 × 10~5 CFU/mL and 6.80 ×10~5 CFU/mL, respectively. More than 90% clones were recombinant and the length of the insert fragments were ranging from400-800 bp in the forward-and reverse-SSH libraries. |
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| Keywords: | Chieh-qua Fusarium wilt suppression subtractive hybridization cDNA library |
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