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| 猪副粘病毒感染的电镜诊断及病毒的分离 | |
| 引用本文： | 周玉,王心蕊,孙磊,张茂林,李金栋,沈庆风,柳增善,池鹤松,吴丹,刘静秋.猪副粘病毒感染的电镜诊断及病毒的分离[J].湖北农业科学,2008,47(9). |
| 作者姓名： | 周玉 王心蕊 孙磊 张茂林 李金栋 沈庆风 柳增善 池鹤松 吴丹 刘静秋 |
| 作者单位： | 1. 吉林大学教育部人兽共患病重点实验室/人兽共患病研究所,长春,130062 2. 吉林省动物疫病预防控制中心,长春,130062 3. 吉林省产品质量监督检测院,长春,130022 |
| 摘 要： | 采用电镜负染技术对长春市某猪场病死仔猪的肠道内容物、肾脏、脾脏、肝脏、肺脏、脑等组织进行了观察.结果在肠道内容物中发现大量副粘病毒,同时在肾脏、肝脏和肺脏中也发现数量不等的副粘病毒,在脾脏和脑组织中未发现病毒粒子.取肾脏为病料,采用同步培养方法接种于PK细胞系进行病毒的分离培养.结果表明,电镜负染技术可对猪感染副粘病毒进行快速诊断,该方法操作程序简便,速度快,从取样到结果判定在1h之内即可完成.细胞分离培养结果证实此病例中的猪副粘病毒可在PK细胞中克隆增殖,并引起细胞典型的病变(CPE). |
| 关 键 词： | 猪 副粘病毒 电镜诊断 病毒培养 |
Electron Microscope Diagnose and Isolation of the Infection of Swine Paramyxovirus |
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| ZHUO Yu,WANG Xin-rui,SUN Lei,ZHANG Mao-lin,LI Jin-dong,SHEN Qing-feng,LIU Zeng-shan,CHI He-song,WU Dan,LIU Jing-qiu.Electron Microscope Diagnose and Isolation of the Infection of Swine Paramyxovirus[J].Hubei Agricultural Sciences,2008,47(9). | |
| Authors: | ZHUO Yu WANG Xin-rui SUN Lei ZHANG Mao-lin LI Jin-dong SHEN Qing-feng LIU Zeng-shan CHI He-song WU Dan LIU Jing-qiu |
| Institution: | ZHUO Yu1,WANG Xin-rui1,SUN Lei1,ZHANG Mao-lin1,LI Jin-dong1,SHEN Qing-feng1,LIU Zeng-shan1,CHI He-song2,WU Dan2,LIU Jing-qiu3 (1.Key Laboratory of Zoonoses,Ministry of Education,Institute of Zoonoses,Jilin University,Changchun 130062,China,2.The Prevention , Regulation Center of Animal Deasease of Jilin Province,3.The Production Quality Inspection Faculty of Jilin Province,Changchun 130022,China) |
| Abstract: | The intestinal contents,kidney spleen liver lung and brain tissues were observed by electron microscope.As the results,abundant paramyxovirus were found in the interior materials of intestine and defferent number of paramyxovirus also been found in kidney liver and lung,and no paramyxovirus were found in spleen and brain tissues.The kidney was collected from dead swine for virus isolation in PK cell culture.A strain of virus was isolated and identified as swine paramyxovirus by morphology serologic test.The... |
| Keywords: | swine paramyxo virus electron microscope diagnose virus incubation |
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