蛙携带虹彩病毒的分子流行病学研究

运用多重实时PCR对多地的养殖和野生蛙类携带虹彩病毒的本底展开调查,并进行病毒分型。结果表明,检测的样品平均阳性率为8.90%,养殖蛙的平均阳性率为4.57%,野生蛙的平均阳性率为16.82%。从蛙种类来看,养殖牛蛙和黑斑蛙带毒率较低,而养殖的棘胸蛙和野外捕捉的斑腿树蛙带毒率较高。不同蛙携带的蛙病毒的分子序列比较结果显示,不同来源的蛙病毒高度保守,缺乏明显的宿主与地域特异性,表明人工繁育场暴发虹彩病毒可能对其他野生蛙类资源产生了负面影响,建议在野生动物人工驯养繁殖体系中引入病害监测和疫病防控措施。

Study on Molecular Epidemiology of Iridoviruses in Rana

The multiplex real-time PCR assay was used to investigate the prevalence of iridoviruses in farmed or wild frog. Results indicated that the average positive rate was 8.90%,in which the average positive rate in farmed and wild frog was 4.57% and 16.82%,respectively. The most significant prevalence of iridoviruses was found among the farmed Quasipaa spinosa and Polypedates megacephalus,while the species of Rana catesbeiana and Rana nigromaculata rarely. Sequences alignement of major capsid protein suggested that there were no obvious host and geographic specificity among these detected Rana iri-doviruses,it indicated that the outburst of iridoviruses in farm produced negative effects on wild frogs,disease monitoring,dis-ease prevention and control measures were proposed to introduce into the artificial domestication propagation system of wild animal.