| 马铃薯Y病毒广东分离物HC-Pro基因的克隆和序列分析 |
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| 引用本文： | 郭小建.马铃薯Y病毒广东分离物HC-Pro基因的克隆和序列分析[J].湖北农业科学,2011(20):4298-4301. |
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| 作者姓名： | 郭小建 |
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| 作者单位： | 仲恺农业工程学院生命科学学院; |
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| 基金项目： | 广东省教育厅优秀青年创新人才培育项目(LYM08065) |
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| 摘 要： | 利用RT-PCR法对广东烟草样品的马铃薯Y病毒(Potato virus Y,PVY)辅助成分蛋白质(Helpercomponent proteinase,HC-Pro)基因进行了克隆,并对其进行了序列分析。测序结果表明广东PVY HC-Pro(命名为PVY-HC-GD)基因(用PVY-HC-GD表示)全长1 395 bp,编码465个氨基酸。与已报道的PVY HC-Pro基因核苷酸序列相比发现,PVY-HC-GD与PVY NTN株系(PVYNTN)(AB331517)的PVYHC-Pro基因核苷酸序列相似性最高,达99.6%。而氨基酸序列比较表明,PVY-HC-GD与PVY N株系(PVYN)(AM268435)的PVY HC-Pro氨基酸序列相似性最高,为99.1%。进一步将PVY-HC-GD的核苷酸和其编码的氨基酸序列与其他报道的同源基因比对构建了系统关系树,结果显示广东烟草样品中PVY-HC-GD与PVYNTN PVY HC-Pro亲缘关系最近。
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| 关 键 词： | 马铃薯Y病毒 广东烟草样品 HC-Pro基因 克隆 序列分析 |
Cloning and Sequence Analysis of HC-Pro of PVY-GD Isolate |
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| GUO Xiao-jian.Cloning and Sequence Analysis of HC-Pro of PVY-GD Isolate[J].Hubei Agricultural Sciences,2011(20):4298-4301. |
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| Authors: | GUO Xiao-jian |
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| Institution: | GUO Xiao-jian(College of Life Sciences,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China) |
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| Abstract: | Helper component proteinase(HC-Pro) gene was cloned by RT-PCR from tobacco leaves infected with Potato virus Y(PVY) in Guangdong,and then sequenced.The full length of HC-Pro of PVY from tobacco in Guangdong(PVY-HC-GD) was 1 395 bp,encoding 465 amino acids.Sequence analysis showed that PVY-HC-GD shared the highest nucleotide sequence similarity with Potato virus Y strain NTN(AB331517)(99.6%),and the highest amino acid sequence similarity with Potato virus Y strain N(AM268435)(99.1%).Phylogenetic trees constr... |
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| Keywords: | potato virus Y tobacco leaves in Guangdong HC-Pro gene clone sequence analysis |
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