蜘蛛香中绿原酸及总酚酸的含量测定

试验建立了蜘蛛香(Valeriana jatamansi Jones)中绿原酸和总酚酸的含量测定方法。利用高效液相色谱法(HPLC)测定蜘蛛香中绿原酸的含量,色谱柱为Dikma C_(18)(4.6 mm×250 mm,5μm),流动相为乙腈-0.4%磷酸水溶液(12∶88),流速1.0 mL/min,检测波长327 nm,柱温30℃;以绿原酸为对照品,采用紫外-可见分光光度法(UV)在327 nm波长处测定蜘蛛香中总酚酸的含量。结果表明,绿原酸在2~20μg/mL范围内浓度与峰面积线性关系良好,r=0.999 6,加样回收率为97.95%,RSD为1.37%;总酚酸在5~20μg/mL范围内浓度与吸光度线性关系良好,r=0.999 8,加样回收率为100.31%,RSD为1.34%。该方法简便、快速、准确、重复性好,可用于蜘蛛香中绿原酸和总酚酸的含量测定。

Determination of Chlorogenic Acid and Total Phenolic Acid Content in Valeriana jatamansi

To establish a method for determining chlorogenic acid and total phenolic acid in Valeriana jatamansi Jones. Chlorogenic acid content in V. jatamansi was determined by high performance liquid chromatography, as the Dikma ODS (4.6 mm×250 mm, 5μm) column was adopted, the mobile phase was acetonitrile-0.4% phosphoric acid(12:88) at the flow rate of 1.0 mL/min, the detection wavelength was 327 nm, and the column temperature was 30℃. Total phenolic acid content in V . jatamansi was determined by ultraviolet-visible spectrophotometry spectrophotometer with chlorogenic acid as standard reference at the detection wavelength of 327 nm. Chlorogenic acid showed good linearity in the range of 2 to 20μg/mL(r=0.9996); the recovery was 97.95%(RSD=1.37%). Total phenolic acid had good linear relationship within the range of 5 to 20μg/mL (r=0.9998); the recovery was 100.31%(RSD=1.34%). The method is simple, rapid, accurate, reproducible and could be used for measuring the chlorogenic acid and total phenolic acid content in V . jatamansi.