| 栽培稻、斑点野生稻、药用野生稻基因组比较分析 |
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| 引用本文： | 高露,王德彬,刘新琼,覃瑞.栽培稻、斑点野生稻、药用野生稻基因组比较分析[J].湖北农业科学,2007,46(4):491-494. |
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| 作者姓名： | 高露 王德彬 刘新琼 覃瑞 |
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| 作者单位： | 中南民族大学生命科学学院生物技术国家民委重点实验室,武汉,430074 |
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| 基金项目： | 中国博士后科学基金
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湖北省自然科学基金 |
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| 摘 要： | 以栽培稻总DNA为探针,对栽培稻(AA)自身、斑点野生稻(BB)以及药用野生稻(CC)体细胞染色体进行基因组荧光原位杂交(GISH),并以斑点野生稻总DNA为探针,对自身和药用野生稻体细胞染色体进行基因组荧光原位杂交,以此研究A、B、C 3个基因组型之间的关系.结果显示,A、B、C基因组之间都存在较高的同源性,其中AA与CC之间的信号最强,BB基因组与AA基因组次之,BB基因组与CC基因组的信号最弱.说明A、B、C 3个基因组之间的亲缘关系,A与C最近,B与C最远.
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| 关 键 词： | 基因组荧光原位杂交(GISH) 栽培稻 斑点野生稻 药用野生稻 |
| 文章编号： | 0439-8114（2007）04-0491-04 |
| 修稿时间： | 2007-05-25 |
Comparative Analysis of Genomes in Oryza Sativa,O. punctata and O. officinalis |
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| GAO Lu,WANG De-bin,LIU Xin-qiong,QIN Rui.Comparative Analysis of Genomes in Oryza Sativa,O. punctata and O. officinalis[J].Hubei Agricultural Sciences,2007,46(4):491-494. |
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| Authors: | GAO Lu WANG De-bin LIU Xin-qiong QIN Rui |
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| Institution: | Key Laboratory of State Ethnic Affairs Commission for Biological Technology, College of Life Sciences South-Central University for Nationalities, Wuhan 430074,China |
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| Abstract: | Genomic in situ hybridization(GISH) was applied to somatic chromosomes preparations of Oryza sativa(AA),O.punctata(BB) and O.officinalsis(CC) with genomic DNA from O.sativa as probes,and to somatic chromosomes preparations of O.punctata(BB) and O.officinalis(CC) with genomic DNA from O.punctata as probes.It was obvious that there were close relationship between A,B and C genomes.The results showed the relationship between A and C genome was the closest,and that of between B and C genome was distant. |
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| Keywords: | GISH O sativa O punctata O officinalis |
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