正交试验法优化甲醇提取艾蒿中槲皮素工艺条件

以艾蒿为原料,槲皮素含量为指标,在单因素试验基础上,采用正交试验设计法优化甲醇提取艾蒿中槲皮素的工艺。结果表明,甲醇提取艾蒿中槲皮素最佳提取工艺为料液比1∶16(g/m L),甲醇体积分数100%,浸泡时间2 h,超声时间30 min。按优选工艺条件下槲皮素含量为0.698 mg/g,验证试验证明结果稳定,具有生产应用价值。

Study on Orthogonal Test Optimization of Methanol Extraction of Quercetin From Artemisia argyi Process Conditions

Artemisiaargyi was treated the raw material with methanolto extract quercetinand the yield of quercetinwas used to be an indicator. On the basis of single-factor experiments,the orthogonal experiment method were carried out to optimize the extraction process ofquercetin. The results showed that the optimal extraction parameters were found as follows:mass fraction of methanol 100%;material-to-liquid 1:16 g/mL;soak time 4 h;ultraphonic time 30 min. According to the optimal process condi-tions the yield ofquercetin was 0.698%.It could be well fitted with model and was reliable and applicable for actual predic-tion.It had value of production.