| 生物酶法提取沙棘果渣总黄酮工艺的优化 |
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| 引用本文： | 徐升运,赵文娟,陈卫锋,任平,秦涛.生物酶法提取沙棘果渣总黄酮工艺的优化[J].湖北农业科学,2012(5):983-986. |
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| 作者姓名： | 徐升运 赵文娟 陈卫锋 任平 秦涛 |
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| 作者单位： | 陕西省科学院酶工程研究所;陕西省酶工程技术中心 |
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| 基金项目： | 中国科学院西部之光项目(2007KDF-05) |
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| 摘 要： | 用生物酶法提取沙棘(Hippophae rhamnoides)果渣中的总黄酮,通过单因素试验和正交试验优化提取工艺。结果表明,酶解体系各因素对总黄酮提取率影响的大小顺序为酶用量、酶解温度、酶解时间、pH,最佳提取条件为生物酶用量占沙棘果渣质量的4%、pH 5、酶解温度60℃、酶解时间120 min,料水质量比1∶30,此条件下总黄酮提取率可达到0.796%。与有机溶剂回流法和超声波法相比,生物酶法所得沙棘果渣的总黄酮提取率分别提高了51.91%和48.51%。
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| 关 键 词： | 生物酶法 沙棘(Hippophae rhamnoides)果渣 总黄酮 提取工艺 |
Optimization of Bio-enzyme Method for Total Flavones Extraction from Marc of Sea Buckthorn |
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| XU Sheng-yun,ZHAO Wen-juan,CHEN Wei-feng,REN Ping,QIN Tao.Optimization of Bio-enzyme Method for Total Flavones Extraction from Marc of Sea Buckthorn[J].Hubei Agricultural Sciences,2012(5):983-986. |
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| Authors: | XU Sheng-yun ZHAO Wen-juan CHEN Wei-feng REN Ping QIN Tao |
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| Institution: | 1.Enzyme Engineering Institute,Shaanxi Academy of Sciences,Xi’an 710600,China; 2.Shaanxi Engineering Center of Enzyme Technology,Xi’an 710600,China) |
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| Abstract: | Bio-enzyme method was applied for total flavones extraction from marc of sea buckthorn(Hippophae rhamnoides);and the extraction craft was optimized by single factor experiments and orthogonal design.The results showed that the optimum extraction conditions for the enzyme reaction system were as follows,enzyme dose,4% of the mass of marc of sea buckthorn;pH,5;Temperature,60 ℃;Time,120 min;Mass ratio of material to water,1∶30.Under the optimum conditions,the yield of total flavones was up to 0.796%.Compared with heat reflux extraction and ultra-sonic extraction,the yield of bio-enzyme extraction was increased by 51.91% and 48.51%,respectively. |
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| Keywords: | bio-enzyme method marc of sea buckthorn(Hippophae rhamnoides) total flavones extraction technology |
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