| 离心萃取-紫外光度法快速测定茶饮料中的咖啡因 |
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| 引用本文： | 信建豪,邢建华,辛媛媛,刘乐,李建业.离心萃取-紫外光度法快速测定茶饮料中的咖啡因[J].湖北农业科学,2012(4):810-812. |
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| 作者姓名： | 信建豪 邢建华 辛媛媛 刘乐 李建业 |
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| 作者单位： | 黄河科技学院医学院 |
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| 摘 要： | 建立以三氯甲烷为提取剂,以离心萃取为提取方法,以276 nm为检测波长的茶饮料中咖啡因的检测方法。结果表明,吸光度A与咖啡因浓度C在5.324～16.940 mg/L呈现良好的线性关系,相关系数为0.999 5,回收率为97.6%～99.4%。该方法测定快速、准确度高,适用于茶饮料中咖啡因的测定。
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| 关 键 词： | 离心萃取 快速测定 咖啡因 茶饮料 |
Centrifugal Extraction and Determination of Caffeine in Tea Drink by UV-Vis Spectrophotometry |
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| XIN Jian-hao,XING Jian-hua,XIN Yuan-yuan,LIU Le,LI Jian-ye.Centrifugal Extraction and Determination of Caffeine in Tea Drink by UV-Vis Spectrophotometry[J].Hubei Agricultural Sciences,2012(4):810-812. |
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| Authors: | XIN Jian-hao XING Jian-hua XIN Yuan-yuan LIU Le LI Jian-ye |
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| Institution: | (School of Medicinal,Huanghe S&T College,Zhengzhou 450063,China) |
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| Abstract: | The UV-Vis method for detecting caffeine in tea drink at 276 nm after centrifugal extraction was established.The results showed that the absorbence(A) showed a good linear relation with concentration of caffeine(C) from 5.324 mg/L to 16.940 mg/L.The correlation coefficient was 0.999 5 and the recovery ranged from 97.6% to 99.4%.In conclusion,the method was suitable for rapid determination of caffeine in tea drink. |
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| Keywords: | centrifugal extraction rapid determination caffeine tea drink |
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