清远市肿眼肉鸡病原诊断和药敏试验

广东省清远地区3群肉鸡临床表现不同程度的肿眼、流鼻水等症状,临床治疗处理效果不理想。在3群病鸡中各取病鸡鼻窦腔分泌液进行细菌分离培养、革兰氏和瑞氏染色,并对分离菌株进行了玻片凝集和生化试验。结果表明,革兰氏染色呈阴性,瑞氏染色呈蓝色,油镜下可见细菌呈短杆菌或球杆菌,两极着色,3群分离株均与副鸡嗜血杆菌A型血清完全凝集反应,与B和C型血清无凝集反应,确定肿眼肉鸡病原为A型副鸡嗜血杆菌。同时选用多种药物研究了该病原菌的药敏性,结果显示,A型副鸡嗜血杆菌对头孢噻呋、氟苯尼考和青霉素3种药物均表现极敏。

The Pathogen Diagnosis and Drug Sensitivity Test of Swollen Eye Chicken from Qingyuan City

Different clinical symptoms of swollen eye and runny nose were shown on three groups of chicken in Qingyuan district,Guangdong Province and ideal clinical treatment result had not achieved.In order to detect the pathogen of these three groups of chickens,bacterial isolation and culture,gram staining and wright stain were performed on nasal fluids taken from them as well as biochemical and slide agglutination tests on relevant isolated bacterial strains.The results showed that the bacteria were gram negative and blue for wright stain,showed the shape of bacilli or cocci under oil immersion objective,bipole stained,could strongly agglutinate with Haemophilus paragallinarum(HPG) serotype A serum but not with serotype B or C serum.Consequenty,the pathogen of this swollen eye chicken was identified as HPG serotype A.Drug sensitivity test was carried out simultaneously on this pathogen and showed that HPG serotype A was super hypersensitive to ceftiofur,florfenicol and penicillin.