| 拮抗木霉菌株T32的紫外线诱变改良 |
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| 引用本文： | 康萍芝,张丽荣.拮抗木霉菌株T32的紫外线诱变改良[J].湖北农业科学,2012(1):44-47. |
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| 作者姓名： | 康萍芝 张丽荣 |
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| 作者单位： | 宁夏农林科学院植物保护研究所/宁夏植物病虫害防治重点实验室 |
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| 基金项目： | 宁夏回族自治区自然科学基金项目(NZ0980) |
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| 摘 要： | 采用紫外线诱变处理与含药(百菌清)培养基驯化相结合的方法,对拮抗木霉菌株T32进行改良,获得了4株在百菌清杀菌剂2 000 mg/L浓度水平下仍能较好生长的抗性菌株,并通过测定其生长速率、产孢量、敏感性、拮抗作用以及遗传稳定性等指标,筛选出了性状优良的抗性菌株T32-5-10m。试验结果表明,经诱变的木霉菌株T32-5-10m与亲本木霉菌株T32相比较,其抗药性提高了20倍,生长速率和产孢能力均高于亲本菌株,连续转接10代,其抗药性十分稳定,且对供试3种土传病原真菌的抑制率达87.56%～89.11%。
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| 关 键 词： | 紫外线诱变 拮抗木霉菌株T32 百菌清 抗药性 |
Improvement of Antagonistic Trichoderma Strain T32 by Ultraviolet Mutation |
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| KANG Ping-zhi,ZHANG Li-rong.Improvement of Antagonistic Trichoderma Strain T32 by Ultraviolet Mutation[J].Hubei Agricultural Sciences,2012(1):44-47. |
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| Authors: | KANG Ping-zhi ZHANG Li-rong |
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| Institution: | (Institute of Plant Protection,Ningxia Academy of Agriculture and Forestry Sciences/Ningxia Key Laboratory for Control of Plant Disease and Insect Pest,Yinchuan 750002,China) |
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| Abstract: | Antagonistic Trichoderma strain T32 was improved by ultraviolet mutation and chlorothalonil medicinal cultivation.4 chlorothalonil resistance strains which could grow well when chlorothalonil concentration was 2 000 mg/L were obtained.A resistance strain T32-5-10m with good features was screened by testing the growth rate,spore production yield,sensitivity,counteraction,and genetic stability.The results showed that compared with parent strain T32,resistance of mutant T32-5-10m was raised by 20 times;growth rate and spore production of T32-5-10m was higher than that of T32.The medical resistance of T32-5-10m was steady after 10 successive transfer cultures,and its inhibition rate against 3 soil diseases pathogens was 87.56%～89.11%. |
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| Keywords: | ultraviolet mutation antagonistic Trichoderma T32 chlorothalonil resistance |
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