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| 辣椒枯萎病生防菌株的筛选与鉴定 | |
| 引用本文： | 杨卫娟,刘长远,马晓飞,李利霞,黄俊生.辣椒枯萎病生防菌株的筛选与鉴定[J].湖北农业科学,2012,51(9):1791-1794,1799. |
| 作者姓名： | 杨卫娟 刘长远 马晓飞 李利霞 黄俊生 |
| 作者单位： | 1. 沈阳农业大学植物保护学院,沈阳,110161 2. 辽宁省农业科学院植物保护研究所,沈阳,110161 3. 中国热带农业科学院环境与植物保护研究所,海南儋州,571737 |
| 基金项目： | 公益性行业(农业)科研专项，辽宁省科技攻关项目，辽宁省设施蔬菜产业创新团队项目 |
| 摘 要： | 从辽宁省不同地区菜地采集土样,采用平板对峙法和抑菌圈法筛选对辣椒枯萎病菌(Fusarium oxysporum)具有拮抗作用的菌株.通过初筛得到具有拮抗作用的菌株12株,复筛出1株拮抗活性较高的菌株SY-17.通过含毒介质法对其发酵滤液进行毒力测定,SY-17发酵滤液EC50为114.50 μL/mL,对辣椒枯萎病菌表现出较好的抑制作用.通过生理生化试验、形态特征观察及16 S rDNA序列分析对其进行了种属鉴定,鉴定结果菌株SY-17属于解淀粉芽孢杆菌(Bacillus amyloliquefaciens). |
| 关 键 词： | 辣椒枯萎病菌(Fusarium oxysporum) 生防菌 形态学观察 分子鉴定 控病机理 |
Separation and Identification of Microbe against Fusarium oxysporum |
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| YANG Wei-juan , LIU Chang-yuan , MA Xiao-fei , LI Li-xia , HUANG Jun-sheng.Separation and Identification of Microbe against Fusarium oxysporum[J].Hubei Agricultural Sciences,2012,51(9):1791-1794,1799. | |
| Authors: | YANG Wei-juan LIU Chang-yuan MA Xiao-fei LI Li-xia HUANG Jun-sheng |
| Institution: | 1.College of Plant Protection,Shenyang Agricultural University,Shenyang 110161,China; 2.Institute of Plant Protection,Liaoning Academy of Agricultural Sciences,Shenyang 110161,China; 3.Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences,Danzhou 571737,Hainan,China) |
| Abstract: | Twelve bacteria strains were isolated by sampling soil from fields of various regions via preliminary screening.A strain named SY-17 with a relatively higher antagonistic activity was obtained via secondary screening.The result showed that the EC50 of strain SY-17 zymosis filtrate was 114.50 μL/mL according to containing poison medium method.Strain SY-17 was characterized by physiological and biochemical experiments,morphological features observation,and 16 S rDNA sequence analysis,based on which it was finally identified as Bacillus amyloliquefaciens. |
| Keywords: | Fusarium oxysporum biocontrol microbe morphological observation molecular identification disease-resistant mechanism |
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