高效液相色谱法测定漆姑草中芦丁和槲皮素含量

建立了漆姑草(Sagina japonica)中芦丁和槲皮素含量的反相高效液相色谱—二极管阵列检测法,采用乙醇—超声波提取,以Shim-pack VP-ODS (200 mm×4.6 mm)为色谱柱,以甲醇∶水(含0.2％磷酸)=60∶40(V/V)为流动相,流速为0.8 mL/min,柱温为30℃,检测波长为255 nm.芦丁和槲皮素能得到较好的分离,分别在0.03～15,00 μg/mL(r=0.999 0)和0.04～20.00 μg/mL(r=0.999 4)的浓度范围内与峰面积成良好线性关系,检测限分别为12.6和15.2 ng/mL.本方法操作简单、准确度高、重现性好,可用于漆姑草药材的质量控制,为漆姑草质量标准的制定提供依据.

Contents of Rutin and Quercetin in Sagina japonica(Sweet) Ohwi Determined by HPLC

To establish a HPLC-PDA method for the determination of rutin and quercetin in Sagina japonica(Sweet) Ohwi,the extraction of rutin and quercetin from S.japonica by ethanol with ultrasound wave was performed.Experiments were performed on C18(Shim-pack VP-ODS 200 mm×4.6 mm) column,with MeOH and H2O(60∶40,V/V) which contained 0.2% phosphoric acid as mobile phase at a flow rate of 0.8 mL/min.The column temperature was 30 ℃ and the determination wavelength was 255 nm.External standard method was used and the calibration curves showed good linearity over the range of 0.03～15.00 μg/mL(r=0.999 0)and 0.04~20.00 μg/mL(r=0.999 4) for rutin and quercetin,respectively.The detection limits were 12.6 and 15.2 ng/mL for rutin and quercetin,respectively.This established method was accurate,reliable,reproducible,and could be used to control the quality of S.japonica and it offered a basis for S.japonica standards.