HPLC-MS测定江油附子中三种生物碱的含量

建立了附子中乌头碱、新乌头碱、次乌头碱的高效液相色谱-质谱联用检测方法,并用该方法测定不同秸杆覆盖处理的附于中3种生物碱成分.以PolarisC18-A(50 mm×2.0 mm,5.0 μm)为色谱柱；甲醇和水(体积比为80∶20)为流动相,流速为0.2 mL/min,检测波长为240 nm,柱温为30℃.结果表明,乌头碱、新乌头碱、次乌头碱分别在0.70～35.00 ng(r=0.999 6)、0.50～25.00 ng (r=0.999 6)、1.06～53.00 ng (r=0.9980)范围内线性良好；平均回收率分别为100.7％、99.2％和98.9％.该方法结果准确可靠,是一种快速灵敏、专属性强的分析方法.

Determination of the Content of Three Aconitum Alkaloids in Radix Aconite Lateralis by HPLC-MS

The high performance liquid chromatography-mass spectrum(HPLC-MS) method was established to determine the effective components in Radix aconite lateralis.The three components in Radix aconite lateralis dealt with different treatments under the cover of straw were determinated.The separation was performed on Polaris C18-A(50 mm×2.0 mm,5.0 μm)column,the mixture of CH3OH-H2O(80:20) was selected as the mobile phase with the flow rate of 0.2 mL/min,the detection wavelength was 240 nm and the column temperature was 30 ℃.The linearity range of three compounds in Radix aconite lateralis was 0.70～35.00 ng(r=0.999 6),0.50～25.00 ng(r=0.999 6),and 1.06～53.00 ng(r=0.998 0),respectively.The average recovery rate was 100.7% for aconitine,99.2% for mesaconitine and 98.9% for hypaconitine.This method was precision and accuracy through the research.