| 耐高温纤维素酶产生菌的筛选鉴定及酶谱分析 |
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| 引用本文： | 张伟,石丽娜,付宁,杨明明,龚月生.耐高温纤维素酶产生菌的筛选鉴定及酶谱分析[J].湖北农业科学,2012,51(9):1767-1770. |
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| 作者姓名： | 张伟 石丽娜 付宁 杨明明 龚月生 |
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| 作者单位： | 西北农林科技大学动物科技学院,陕西杨凌,712100 |
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| 摘 要： | 采用稀释平板-刚果红染色法从腐木中分离到1株产耐高温纤维素酶的菌株CY15-10.通过形态观察、特征培养及16 S rDNA序列分析,鉴定该菌株为多粘类芽孢杆菌(Paenibacillus polymyxa).该菌株经摇瓶发酵后测定粗酶液CMC酶活为1.27 U/mL,最适反应温度为55℃,热稳定性较好.经聚丙烯酰胺凝胶电泳(SDS-PAGE)及酶谱分析表明该菌株向胞外分泌1种纤维素酶组分,酶分子质量约为35 ku.
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| 关 键 词： | 纤维素酶 耐高温 筛选 酶谱分析 细菌 |
Screening, Identiafication and Zymographic Analysis of A Hyperthermophilic Cellulase-producing Strain |
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| ZHANG Wei
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SHI Li-na
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FU Ning
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YANG Ming-ming
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GONG Yue-sheng.Screening, Identiafication and Zymographic Analysis of A Hyperthermophilic Cellulase-producing Strain[J].Hubei Agricultural Sciences,2012,51(9):1767-1770. |
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| Authors: | ZHANG Wei SHI Li-na FU Ning YANG Ming-ming GONG Yue-sheng |
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| Institution: | (College of Animal Science,Northwest A&F University,Yangling 712100,Shaanxi,China) |
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| Abstract: | A hyperthermophilic cellulase-producing strain CY15-10 was isolated from decayed wood and compost through the congo red medium plate method.Based on morphology,biochemical and physiological characterization,16 S rRNA sequence analysis and construction of phylogenetic tree,CY15-10 strain was identified as Paenibacillus polymyxa.The CMCase was 1.27 U/mL.The enzyme had the highest activity when temperature was 55 ℃ and performed good thermal stability.The SDS-PAGE and zymographic analysis detected that a peptide with molecular mass of 35 ku was excretived. |
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| Keywords: | cellulase high-temperature resistance isolation zymographic analysis bactetia |
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