蚕豆锈病的离体叶片人工接种技术研究及微生物源先导化合物筛选

蚕豆锈病由蚕豆单孢锈菌(Uromyces fabae de Bary)引起,是一种常见专性寄生的真菌病害,其侵染和传播方式与大豆锈病(Phakopsora pachyrhiri Syd.)相同,主要以夏孢子传播.因此常作为大豆锈病研究的模式靶标.又因其无法在人工培养基上生长繁殖,而基于植株的筛选方式难以适应先导化舍物筛选.该文针对蚕豆锈病离体叶片人工接种技术进行了探索,对影响蚕豆锈病侵染的主要因素进行了研究,建立了基于离体叶片法筛选的先导化合物筛选方法.采用离体叶片孢子悬浮液喷雾法对1 522份真菌固体发酵提取物和放线菌液体摇瓶发酵提取物进行了初筛,筛选出具有杀菌活性的提取物90份.对经过HPLC纯化后的540个组分进行复筛,获得具较高杀菌活性的化合物3个. 筛选结果表明该筛选方法能稳定、高效地进行先导化合物筛选.

Studies on Artificial Inoculation of Broadbean Rust on Leaf-dish and Screening of Lead Compounds from Microorganism

Broadbean rust is a kind of obhgateparasite.The methods of infecting and spreading are similar with soybean rust (Phakopsora pochyrhizi syd).So it is always used as the model target of soybean rust.The culture of this pathogen is not progressing in media,so the screening of lead compounds confined in plants.The artificial inoculation of broadbean rust had been studied on leaf-dish.The factors of effect on infection of broadbean rust had been studied.The program of screening method was found based on method of the leaf-dish. 1 522 extracts from microorganism had been tested by the screening method,90 extracts showed fungicidal activity.540 samples formulated from profiling by HPLC had been tested,and their lead compounds had been found.The screening results showed that the screening method Was stable and efficient.