| 亚砷酸氧化菌Sinorhizobium sp. GW3的鉴定与亚砷酸氧化酶基因的分离 |
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| 引用本文： | 汪耀,涂书新,王革娇.亚砷酸氧化菌Sinorhizobium sp. GW3的鉴定与亚砷酸氧化酶基因的分离[J].湖北农业科学,2010,49(1). |
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| 作者姓名： | 汪耀 涂书新 王革娇 |
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| 作者单位： | 1. 华中农业大学,农业微生物学国家重点实验室/生命科学技术学院,武汉,430070
2. 华中农业大学,资源与环境学院,武汉,430070 |
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| 基金项目： | 国家自然科学基金项目，教育部博士点基金项目 |
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| 摘 要： | 亚砷酸氧化细菌能够将毒性大的As(Ⅲ)氧化成毒性小的As(Ⅴ),在生物修复砷污染方面具有应用价值。对一株新型亚砷酸氧化菌Sinorhizobium sp.GW3进行了较全面的鉴定,并分离及分析了亚砷酸氧化酶基因aoxAB。形态学、生理生化鉴定和16S rRNA基因等分析结果表明该菌为Sinorhizobium属。该菌对As(III)抗性的MIC(Minimum inhibitory concentration)为9 mmol/L。首次在Sinorhizobium属中分离了包括编码小亚基aoxA和大亚基aoxB在内的亚砷酸氧化酶基因,其编码的大小亚基与已发现的Agrobacterium tumefaciens(ABB51929)的大小亚基在氨基酸水平上分别有86%、80%的同源性。
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| 关 键 词： | 砷 亚砷酸氧化菌 亚砷酸氧化酶基因 反向PCR |
Identification of A Novel Arsenite-Oxidizing Bacterium Sinorhizobium sp.GW3 and Isolation of Arsenite Oxidase Gene |
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| WANG Yao,TU Shu-xin,WANG Ge-jiao.Identification of A Novel Arsenite-Oxidizing Bacterium Sinorhizobium sp.GW3 and Isolation of Arsenite Oxidase Gene[J].Hubei Agricultural Sciences,2010,49(1). |
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| Authors: | WANG Yao TU Shu-xin WANG Ge-jiao |
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| Abstract: | In this stuay,a novel arsenite-oxidizing bacterium GW3 was identified and its arsenite oxidase gene aoxAB was isolated.Based on the morphological, physiological/biochemical characteristics and 16S rRNA gene sequence analyses,this strain was identified as Sinorhizobium sp.GW3.The MIC(Minimum Inhibitory Concentration)for arsenite was 9 mmol/L.This was the first report to isolate full length arsenite oxidase gene including aoxA and aoxB from Sinorhizobium.The aoxA and aoxB of Sinorhizobium sp.GW3 showed 80%and 86%amino acid identifies with the aoxA and aoxB of Agrobacterium tumefaciens(ABB51929),respectively. |
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| Keywords: | Sinorhizobium arsenic arsenite-oxidizing bacterium arsenite oxidase gene reverse PCR Sinorhizobium |
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