| 大孔吸附树脂精制栀子黄色素的梯度洗脱和拐点控制 |
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| 引用本文： | 廖湘萍,林英,李世杰,肖传英.大孔吸附树脂精制栀子黄色素的梯度洗脱和拐点控制[J].湖北农业科学,2009,48(10). |
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| 作者姓名： | 廖湘萍 林英 李世杰 肖传英 |
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| 作者单位： | 1. 湖北轻工职业技术学院,武汉,430070
2. 湖北工业大学发酵工程省部共建教育部重点实验室,武汉,430068 |
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| 摘 要： | 筛选了应用较广的4种大孔吸附树脂YW、NKA、D3520、HPD-100A进行栀子黄色素的吸附分离精制,研究了精制过程中水和低度乙醇洗脱除杂的拐点控制,结合采用反梯度洗脱工艺,得到栀子黄色素产品分别在440nm、325nm和262nm有吸收峰,计算其吸光度比值A325nm/A440nm=0.187,A283nm/A440nm=0.000,A262nm/A440nm=0.220,产品精制回收率皆可超过90%.
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| 关 键 词： | 栀子黄色素 大孔树脂 拐点控制 梯度洗脱 反梯度洗脱 |
The Gardenia Yellow Refined through the Grads Elution and the Inflection Point Control of the Macropore Absorption Resin |
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| LIAO Xiang-ping,LIN Ying,LI Shi-jie,XIAO Chuan-ying.The Gardenia Yellow Refined through the Grads Elution and the Inflection Point Control of the Macropore Absorption Resin[J].Hubei Agricultural Sciences,2009,48(10). |
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| Authors: | LIAO Xiang-ping LIN Ying LI Shi-jie XIAO Chuan-ying |
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| Abstract: | Four macropore resins were selected for gardenia yellow refining by absorption and elution to remove impure matter. The water elution turn point and lower degree ethenol elution turn point were studied and controlled. Combined with reverse grads elution technology,the gardenia yellow product had refined with A_(325nm)/A_(440nm)=0.187,A_(238nm)/A_(440nm)=0,A_(262nm)/A_(440nm)= 0.220. The product recovery of refining was higher than 90%. |
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| Keywords: | gardenia yellow macropore absorption resin inflection point control grads elution reverse grads elution |
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