| 高含量原粉生产菌株苏云金芽孢杆菌LX-7的研究 |
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| 引用本文： | 廖先清,万中义,刘芳,周荣华,杨自文.高含量原粉生产菌株苏云金芽孢杆菌LX-7的研究[J].湖北农业科学,2009,48(12). |
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| 作者姓名： | 廖先清 万中义 刘芳 周荣华 杨自文 |
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| 作者单位： | 湖北省生物农药工程研究中心/湖北省农业科技创新中心生物农药研究分中心,武汉,430064 |
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| 基金项目： | 湖北省重点科技攻关项目，湖北省农业科技创新中心资助项目 |
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| 摘 要： | 从土壤中分离的苏云金芽孢杆菌LX-7高毒力菌株,利用单因子筛选和正交试验,得到其最佳培养基配方(g/L)为:玉米淀粉30,氮源A 30,氮源D 15,氮源E 15,并对其发酵工艺和后处理工艺进行了优化,40 000 L发酵罐发酵制得原粉效价达到80 000 IU/mg以上,晶体蛋白含量达到13.2%.
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| 关 键 词： | 苏云金芽孢杆菌LX-7 工艺优化 |
Study on the Strain LX-7 of Bacillus thuringiensis with High Level of Insecticidal Crystal Protein |
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| LIAO Xian-qing,WAN Zhong-yi,LIU Fang,ZHOU Rong-hua,YANG Zi-wen.Study on the Strain LX-7 of Bacillus thuringiensis with High Level of Insecticidal Crystal Protein[J].Hubei Agricultural Sciences,2009,48(12). |
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| Authors: | LIAO Xian-qing WAN Zhong-yi LIU Fang ZHOU Rong-hua YANG Zi-wen |
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| Abstract: | The high toxicity strain of Bacillus thuringiensis LX-7 isolated from soil was studied in this paper.The optimum formula of the culture medium which containing 30 g/L corn starch,30 g/L nitrogen source A,15 g/L nitrogen source D and 15 g/L nitrogen source E was obtained from unifaetor experiments and orthogonal experiments.Under the optimized conditions of fermentation and post-treatment,the titer of powder and content of insecticidal crystal protein produced in 40 m~3 fermentor had reached 80 000IU/mg and 13.2% respectively. |
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| Keywords: | strain LX-7 of Bacillus thuringiensis process optimization |
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